Psillakis T, Tsatsakis A M, Christodoulou P, Michalodimitrakis M, Paritsis N, Helidonis E
Laboratory of Toxicology, Medical School, University of Crete, Iraklion, Voutes, Greece.
J Clin Pharmacol. 1999 Jan;39(1):55-67. doi: 10.1177/00912709922007561.
Carbamazepine (CBZ) concentrations were determined in the sections of head hair from 40 patients (22 males and 18 females), ages 5 to 81, who were receiving this drug systemically. Hair treatment included dissolution, liquid phase extraction procedures, and immunoassay (Abbott TDx) or high-pressure liquid chromatography (HPLC) analytical techniques. The mean values of CBZ levels in the hair from the 1st section (close to hair root) to the 5th section for female patients were 26.82, 19.18, 17.28, 15.09, and 14.62 micrograms/g according to HPLC measurements. Immunoassay gave generally slightly higher results. The mean values of CBZ in the hair sections according to the immunoassay technique were 30.53, 21.90, 19.83, 17.45, and 16.99 micrograms/g, respectively, from the 1st to the 5th sections. The corresponding mean values for male patients by HPLC and immunoassay techniques were 21.97, 17.30, 15.03, 13.02, and 11.21 micrograms/g and 25.98, 20.52, 17.15, 14.87, and 12.31 micrograms/g. Generally, a reduction of drug concentrations in hair from the first to the subsequent segments was observed. Higher amounts of CBZ were deposited in black, untreated hair (e.g., not dyed or permed). CBZ concentrations in hair sections were found to be dependent on the dosage (r = 0.979, p < or = 0.001) but not on the gender. The data indicate the possible use of hair section testing as a marker of the dosage history and the compliance of patients under long-term treatment with CBZ.
对40例年龄在5至81岁、正在全身接受卡马西平（CBZ）治疗的患者（22名男性和18名女性）的头发样本进行了CBZ浓度测定。头发处理包括溶解、液相萃取程序以及免疫测定（雅培TDx）或高压液相色谱（HPLC）分析技术。根据HPLC测量，女性患者从第1段（靠近发根）到第5段头发中CBZ水平的平均值分别为26.82、19.18、17.28、15.09和14.62微克/克。免疫测定的结果总体上略高。根据免疫测定技术，从第1段到第5段头发中CBZ的平均值分别为30.53、21.90、19.83、17.45和16.99微克/克。男性患者通过HPLC和免疫测定技术得到的相应平均值分别为21.97、17.30、15.03、13.02和11.21微克/克以及25.98、20.52、17.15、14.87和12.31微克/克。一般来说，观察到头发中药物浓度从第一段到后续段逐渐降低。更多的CBZ沉积在黑色、未处理的头发中（例如，未染色或烫发）。发现头发段中的CBZ浓度取决于剂量（r = 0.979，p≤0.001），但与性别无关。数据表明头发段检测可能作为长期接受CBZ治疗患者剂量史和依从性的标志物。
