Kimura N, Kozaki A, Sasaki T, Komatsubara A
Ishikawa Health Service Association.
Kansenshogaku Zasshi. 1999 Jan;73(1):53-61. doi: 10.11150/kansenshogakuzasshi1970.73.53.
To investigate the isolation frequency of O-serotype of Escherichia coli, a total of 1,563 faecal specimens obtained from patients with sporadic diarrhea in Ishikawa between July 1997 and June 1998, were examined. As a result of O-serotyping of isolated strains using commercially E. coli antisera (43 different types), 247 strains of 29 different O-serotypes were isolated. Isolation rate was 15.8%. Most predominant O-serotype was O1 (128 strains, 52%), followed by O18 (26 strains, 11%), O6 (17 strains, 7%), O111 (16 strains, 6%), and these 4 different O-serotypes took up three quarters of the isolated E. coli. Between August 1996 and May 1997, E. coli isolation from faecal samples of 51,893 healthy persons and O-serotyping of isolated strains using commercial antisera to 6 predominant O-serotypes (O-26, 111, 114, 128, 157 and O1) of VTEC/EHEC were carried out. Among 6 O-serotypes, the most predominant O-serotype was O1 (93% of isolates), followed by O26, 111, 128 (6%) and O114, 157 (1%). These isolation frequencies in patients were 80%, 18%, 2%, respectively, have resembled each other in healthy persons in many points. In a similar way, of these distributions of O-serotype of strains hemolysed on Beutin's blood agar plates, we compared patients with healthy persons. Fifty-six strains (3.6% of the total) of E. coli of different O-serotypes were isolated from 1,563 patients and 57 strains (2.8% of the total) belonging to 11 serotypes from 2,036 healthy persons. As a result of O-serotype frequency, both groups resembled each other. O18 and O6, the most predominant O-serotypes, occupied 64% of the isolated strains in patients and 74% in healthy persons. Next in patients, O1, 26 were 7%--level, O28 ac, 152, 157 were 4%--level, respectively, and in healthy persons. O1 was 5%--level, O28 ac, 55, 146, 152 were 4%--level respectively. In the comparison of O-serotype frequency of E. coli isolated from sporadic diarrhea in other 5 areas (Kanto district, Tokyo, Oita, Aichi and Ishikawa), O1, 6, 8, 18, 25, 26, 55, 86a, 111, 125, 126, 127a, 128, 146, 148, 157 and 166 (17 types) have covered a wide area. On the other hand, O29, 44, 78, 112ac, 115, 136, 143, 152, 168 and 169 (10 types) have a tendency to distribute in local areas, we believe that there are regional differences even in the same Japanese territory.
为调查大肠杆菌O血清型的分离频率，对1997年7月至1998年6月期间从石川县散发性腹泻患者中采集的1563份粪便标本进行了检测。使用市售大肠杆菌抗血清（43种不同类型）对分离菌株进行O血清型鉴定，共分离出29种不同O血清型的247株菌株。分离率为15.8%。最主要的O血清型是O1（128株，占52%），其次是O18（26株，占11%）、O6（17株，占7%）、O111（16株，占6%），这4种不同的O血清型占分离出的大肠杆菌的四分之三。1996年8月至1997年5月，对51893名健康人的粪便样本进行大肠杆菌分离，并使用针对6种主要O血清型（O-26、111、114、128、157和O1）的商业抗血清对分离菌株进行O血清型鉴定，这些血清型属于产志贺毒素大肠杆菌/肠出血性大肠杆菌。在这6种O血清型中，最主要的O血清型是O1（占分离株的93%），其次是O26、111、128（占6%）和O114、157（占1%）。这些血清型在患者中的分离频率分别为80%、18%、2%，在很多方面与健康人相似。同样，对于在Beutin血琼脂平板上溶血的菌株的O血清型分布，我们比较了患者和健康人。从1563名患者中分离出56株（占总数的3.6%）不同O血清型的大肠杆菌，从2036名健康人中分离出57株（占总数的2.8%）属于11种血清型的大肠杆菌。从O血清型频率来看，两组相似。最主要的O血清型O18和O6在患者分离菌株中占64%，在健康人中占74%。在患者中其次是O1、26占7%水平，O28ac、152、157占4%水平，在健康人中，O1占5%水平，O28ac、55、146、152占4%水平。在比较从其他5个地区（关东地区、东京、大分、爱知和石川）散发性腹泻患者中分离的大肠杆菌的O血清型频率时，O1、6、8、18、25、26、55、86a、111、125、126、127a、128、146、148、157和166（17种类型）分布广泛。另一方面，O29、44、78、112ac、115、136、143、152、168和169（10种类型）有在局部地区分布的倾向，我们认为即使在日本同一地区也存在区域差异。
