Kobayashi I, Osawa H, Harada T, Saika T, Kanayama A, Muraoka H, Uchino U, Hasegawa M, Sato Y, Numata K, Hashiguchi N, Nishida M
Department of Clinical Microbiology, Mitsubishi Kagaku Bio Clinical Laboratory, Inc.
Kansenshogaku Zasshi. 1997 Jun;71(6):495-500. doi: 10.11150/kansenshogakuzasshi1970.71.495.
A total of 21,609 faecal specimens obtained from patients with diarrhea mainly in Kanto area between June and September 1996 were investigated to identify the causative pathogens for diarrhea. One-hundred fifty-seven strains of Escherichia coli of 29 different O-serotypes were isolated as the causative pathogens, which were previously recognized to induce severe abdominal cramps and diarrhea. Of these, 114 strains, in which the possibility of enterohemorrhagic E. coli due to their O-serotypes was predicted, were examined for the producibility of Vero toxins. Twenty-six (76.5%) of the 34 strains of E. coli O157 produced the Vero toxins, and other 8 strains were the non-producers. Twenty of the 26 producers produced both VT1 and VT2, whereas the other 6 strains produced VT2 only. Furthermore, 4 strains of E. coli O26, and 1 strain each of E. coli O125 and O126 produced Vero toxins. Thirty-two of the 114 strains, isolated from the patients with diarrhea and selected as the enterohemorrhagic E. coli according to the specific O-serotypes, were actually confirmed produce Vero toxins. Thirty-four strains of E. coli O157 tested were susceptible to all antibiotics such as ampicillin, doxycylin, levofloxacin, fosfomycin, chloramphenicol and polymyxin B, and no strains resistant to levofloxacin, polymyxin B and fosfomycin were found.
对1996年6月至9月间主要从关东地区腹泻患者获取的总共21,609份粪便标本进行了调查，以确定腹泻的致病病原体。分离出157株29种不同O血清型的大肠杆菌作为致病病原体，这些病原体以前被认为会引起严重的腹部绞痛和腹泻。其中，预测有114株因其O血清型而可能为肠出血性大肠杆菌，对其Vero毒素的产生能力进行了检测。34株大肠杆菌O157中有26株（76.5%）产生Vero毒素，另外8株不产生。26株产生毒素的菌株中有20株同时产生VT1和VT2，而另外6株仅产生VT2。此外，4株大肠杆菌O26以及各1株大肠杆菌O125和O126产生Vero毒素。从腹泻患者中分离出的114株菌株，根据特定的O血清型被选为肠出血性大肠杆菌，其中32株实际被证实产生Vero毒素。检测的34株大肠杆菌O157对氨苄西林、强力霉素、左氧氟沙星、磷霉素、氯霉素和多粘菌素B等所有抗生素均敏感，未发现对左氧氟沙星、多粘菌素B和磷霉素耐药的菌株。
