Davidson B, Goldberg I, Gotlieb W H, Lerner-Geva L, Ben-Baruch G, Agulansky L, Novikov I, Kopolovic J
Department of Pathology, Sheba Medical Center, Tel Hashomer, Israel.
Acta Obstet Gynecol Scand. 1999 Mar;78(3):240-4.
The role of angiogenesis and inflammatory cell response in predicting disease outcome was evaluated in various malignant tumors. However, the data relating to cervical cancer remains equivocal. This study evaluates the prognostic significance of microvessel counts and peritumoral macrophage infiltrates in squamous cell carcinoma of the uterine cervix.
Seventy-five cervical squamous cell carcinomas were stained immunohistochemically by two endothelial markers- anti-CD31 and Ulex Europaeus lectin I (UEA-I), and the macrophage- specific marker anti-CD68. Microvessel and macrophage counts were performed using a grid at X200 and X400 magnification, respectively, in areas of maximal density ('hot spots'). Five fields were scanned. Microvessel counts were correlated with macrophage density, and both were correlated with patient age, tumor stage, histological grade, and survival.
Microvessel counts were comparable for ulex lectin (mean 6.8+/-4.8/field) and CD31 (8.7+/-5.3/field), and results by both markers correlated (p<0.001). Counts by both markers correlated with tumor stage, being higher in stages Ib-II compared to stage III-IV tumors (p<0.05). No correlation with age, grade, or survival was found. Macrophage counts (mean 13.1+/-12.3 cells/field) did not correlate with any of the clinical parameters studied or with microvessel counts.
Microvessel counts and macrophage density do not correlate with survival in cervical cancer. Neither do they appear to be inter-related. The association between elevated microvessel counts and localized disease may reflect peak angiogenic stimuli by neoplastic cells. We hypothesize that the beneficial role of macrophages in cellular immunity may be opposed by the elaboration of growth factors in the vicinity of neoplastic cells.
血管生成和炎症细胞反应在预测多种恶性肿瘤疾病转归中的作用已得到评估。然而，有关宫颈癌的数据仍不明确。本研究评估微血管计数和肿瘤周围巨噬细胞浸润在子宫颈鳞状细胞癌中的预后意义。
75例宫颈鳞状细胞癌采用两种内皮细胞标志物——抗CD31和欧洲荆豆凝集素I（UEA-I）以及巨噬细胞特异性标志物抗CD68进行免疫组织化学染色。分别在最大密度区域（“热点”）使用网格在X200和X400放大倍数下进行微血管和巨噬细胞计数。扫描五个视野。微血管计数与巨噬细胞密度相关，二者均与患者年龄、肿瘤分期、组织学分级和生存率相关。
荆豆凝集素的微血管计数（平均6.8±4.8/视野）与CD31（8.7±5.3/视野）相当，两种标志物的结果相关（p<0.001）。两种标志物的计数均与肿瘤分期相关，Ib-II期肿瘤的计数高于III-IV期肿瘤（p<0.05）。未发现与年龄、分级或生存率相关。巨噬细胞计数（平均13.1±12.3个细胞/视野）与所研究的任何临床参数或微血管计数均无相关性。
微血管计数和巨噬细胞密度与宫颈癌的生存率无关。它们之间似乎也没有相互关系。微血管计数升高与局限性疾病之间的关联可能反映了肿瘤细胞的血管生成刺激高峰。我们推测，巨噬细胞在细胞免疫中的有益作用可能被肿瘤细胞附近生长因子的产生所抵消。
