Wiggins D L, Granai C O, Steinhoff M M, Calabresi P
Department of Obstetrics and Gynecology, Brown University School of Medicine, Women and Infants' Hospital, Providence, Rhode Island 02905, USA.
Gynecol Oncol. 1995 Mar;56(3):353-6. doi: 10.1006/gyno.1995.1062.
Angiogenesis, the induction of new capillaries and venules, has been associated with tumor growth. Increased tumor size and new vessel growth may further the opportunity for tumor cells to enter the circulation and potentiate metastatic disease. To investigate if tumor angiogenesis could serve as a prognostic factor in cervical carcinoma, we counted microvessels (capillaries and venules) in 29 patients with squamous cell carcinoma of the cervix. Surgical specimens were stained for endothelial cells specifically with Factor VIII to identify all vessels. The microvessels were counted by light microscopy (per 200 x field) in tumor sections with the highest population of microvessels. This was performed by two investigators without knowledge of patient outcome or extent of disease. Microvessel counts in patients with squamous cell carcinoma were significantly different from those of control subjects: 56 +/- 28.9 and 16.3 +/- 3.3 (P = 0.013). There was no correlation between microvessel count and node status, parametrial involvement, depth of invasion, or gross disease. Microvessel count was significantly correlated with vascular space involvement (P = 0.017). Four patients who developed recurrent disease within 1 year had high microvessel counts and yet were node negative and VSI negative at surgery. As shown by Folkman in breast cancer, angiogenesis may also be an independent predictor for recurrent disease in squamous cell carcinoma of the cervix. Microvessel counts could be of prognostic value in patients who do not have other risk factors for disease recurrence.
血管生成，即诱导新的毛细血管和小静脉形成，与肿瘤生长有关。肿瘤大小增加和新血管生长可能会增加肿瘤细胞进入循环系统并促进转移性疾病的机会。为了研究肿瘤血管生成是否可作为宫颈癌的预后因素，我们对29例宫颈鳞状细胞癌患者的微血管（毛细血管和小静脉）进行了计数。手术标本用因子VIII特异性地对内皮细胞进行染色以识别所有血管。在微血管数量最多的肿瘤切片中，通过光学显微镜（每200倍视野）对微血管进行计数。这由两名研究人员进行，他们不知道患者的预后或疾病范围。鳞状细胞癌患者的微血管计数与对照组显著不同：分别为56±28.9和16.3±3.3（P = 0.013）。微血管计数与淋巴结状态、宫旁组织受累情况、浸润深度或大体疾病之间无相关性。微血管计数与血管间隙受累情况显著相关（P = 0.017）。4例在1年内出现复发疾病的患者微血管计数较高，但手术时淋巴结阴性且VSI阴性。正如福克曼在乳腺癌研究中所表明的，血管生成也可能是宫颈鳞状细胞癌复发疾病的一个独立预测因素。对于没有其他疾病复发危险因素的患者，微血管计数可能具有预后价值。
