Paajanen H, Haapasalo H, Kotilainen E, Aunapuu M, Kettunen J
Department of Surgery, Kuopio University Hospital, Finland.
J Spinal Disord. 1999 Feb;12(1):57-60.
To investigate the regenerative potential of human disc tissue, the disc samples were obtained during surgery from 24 adult patients with first lumbar prolapses and from 14 patients with recurrent lumbar prolapses. Preoperative magnetic resonance imaging (MRI) confirmed prolapse and disc degeneration in all cases. The proliferation activity of the sampled connective tissue cells was studied with the immunohistochemical expression of Ki-67(MIB-1) antigen. The antigen was positive in 6 of 24 (25%) specimens from first prolapse and in none of the 14 specimens from the recurrent prolapse. The amount of proliferative cells did not correlate to the degree of disc degeneration in MRI. Our results indicate that connective tissue cells in adult degenerative disc may show proliferation activity after the first herniation and thus regenerative potential. The enhanced matrix proliferation may not be a significant reason for recurrent prolapses because none of the recurrent disc specimen showed proliferation activity.
为了研究人类椎间盘组织的再生潜力，在手术过程中从24例患有第一腰椎间盘突出症的成年患者和14例复发性腰椎间盘突出症患者身上获取了椎间盘样本。术前磁共振成像（MRI）证实所有病例均存在椎间盘突出和退变。通过Ki-67（MIB-1）抗原的免疫组化表达研究了所取结缔组织细胞的增殖活性。在首次突出的24个标本中有6个（25%）抗原呈阳性，而复发性突出的14个标本中无一例阳性。增殖细胞的数量与MRI中椎间盘退变程度无关。我们的结果表明，成人退变椎间盘中的结缔组织细胞在首次突出后可能表现出增殖活性，从而具有再生潜力。基质增殖增强可能不是复发性突出的重要原因，因为复发性椎间盘标本中无一例显示增殖活性。
