Abiko T, Abiko A, Ishiko S, Takeda M, Horiuchi S, Yoshida A
Department of Ophthalmology, Asahikawa Medical College, 4-5 Nishikagura, Asahikawa, 078-8510, Japan.
Exp Eye Res. 1999 Mar;68(3):361-6. doi: 10.1006/exer.1998.0615.
Autofluorescence and advanced glycation end product (AGE) levels were measured in the lenses of 9 diabetic Chinese hamsters and 6 age-matched controls. Lens autofluorescence also was measured in 37 diabetic patients and 14 age-matched controls. Lens autofluorescence values were measured noninvasively with a lens measurement system using color filters with peak transmission at 365- and 434-nm wavelengths (excitation and emission, respectively) that are characteristic of AGE fluorescence. The peak lens autofluorescence level was used as the lens autofluorescence value, and the mean lens autofluorescence values from both eyes of each subject were used for statistical analysis. The AGE levels in one lens from each hamster were measured by noncompetitive enzyme-linked immunosorbent assay with a polyclonal anti-AGE antibody. We found a 2.2 times increase of the mean lens autofluorescence value of diabetic hamsters in comparison with that of controls (P<0.01). We also found a 1.5 times increase of the mean AGE level from the lenses of diabetic hamsters in comparison with that of controls (P<0.01). Moreover, a statistically significant positive correlation between the AGE level and autofluorescence value in the same lenses was observed in all hamsters (rho=0.58, P<0.05). In human subjects, we found a 1.4 times increase of the mean lens autofluorescence value of diabetic patients in comparison with that of age-matched controls (P<0.01). Our results suggest that non invasive measurement of lens autofluorescence may be a guide to AGE levels in lenses.
在9只患有糖尿病的中国仓鼠和6只年龄匹配的对照仓鼠的晶状体中测量了自发荧光和晚期糖基化终产物（AGE）水平。还在37名糖尿病患者和14名年龄匹配的对照者中测量了晶状体自发荧光。使用具有365纳米和434纳米波长（分别为激发和发射波长）峰值透射率的滤色片，通过晶状体测量系统对晶状体自发荧光值进行无创测量，这些波长是AGE荧光的特征。将晶状体自发荧光峰值水平用作晶状体自发荧光值，并将每个受试者双眼的平均晶状体自发荧光值用于统计分析。通过使用多克隆抗AGE抗体的非竞争性酶联免疫吸附测定法测量每只仓鼠一个晶状体中的AGE水平。我们发现，与对照组相比，糖尿病仓鼠的平均晶状体自发荧光值增加了2.2倍（P<0.01）。我们还发现，与对照组相比，糖尿病仓鼠晶状体的平均AGE水平增加了1.5倍（P<0.01）。此外，在所有仓鼠中，同一晶状体的AGE水平与自发荧光值之间存在统计学上显著的正相关（rho=0.58，P<0.05）。在人类受试者中，我们发现与年龄匹配的对照组相比，糖尿病患者的平均晶状体自发荧光值增加了1.4倍（P<0.01）。我们的结果表明，晶状体自发荧光的无创测量可能是晶状体中AGE水平的一个指标。
