Silva J, Freitas J P, Filipe P, Vendrell E, Rodrigo F G
Serviço de Oftalmologia do Hospital de Santo António dos Capuchos, Lisboa.
Acta Med Port. 1996 Jul-Sep;9(7-9):237-9.
A body of evidence suggests that glycation of proteins and the resulting fluorescent products take part in the late complications of diabetes. The purpose of this study was to analyse the fluorescence of lens soluble proteins from diabetic patients. Soluble proteins were obtained from lens with cataract from 20 type II diabetic patients and from 21 non diabetic controls with similar age. The fluorescence of the soluble fraction (EX-360 nm, EM -454 nm) was quantified as a parameter for glycation end products. The glycation of plasma proteins was quantified by fructosamine levels. The fluorescence mean in the group of diabetics was about twice the non diabetic value (p < 0.01). The fluorescence in the diabetics with retinopathy was higher than that of diabetic without retinopathy (p < 0.01). Fructosamine levels were: diabetics 3.1 +/- 1.1 mM, non diabetics 1.9 +/- 0.8 mM (p < 0.001). The results suggest the involvement of fluorescent products, resulting from glycation of proteins, in lens opacification in diabetic patients.
大量证据表明，蛋白质糖基化及由此产生的荧光产物参与了糖尿病的晚期并发症。本研究的目的是分析糖尿病患者晶状体可溶性蛋白的荧光。从20例II型糖尿病患者的白内障晶状体以及21例年龄相仿的非糖尿病对照者的晶状体中获取可溶性蛋白。将可溶性部分的荧光（激发波长360nm，发射波长454nm）定量，作为糖基化终产物的一个参数。通过果糖胺水平对血浆蛋白糖基化进行定量。糖尿病组的荧光平均值约为非糖尿病组的两倍（p<0.01）。患有视网膜病变的糖尿病患者的荧光高于无视网膜病变的糖尿病患者（p<0.01）。果糖胺水平为：糖尿病患者3.1±1.1mM，非糖尿病患者1.9±0.8mM（p<0.001）。结果表明，蛋白质糖基化产生的荧光产物参与了糖尿病患者晶状体混浊的形成。
