Olsson T G, Silverstein R A, Ekwall K, Sunnerhagen P
Department of Molecular Biology, Lundberg Laboratory, Göteborg University, P. O. Box 462, S-405 30 Göteborg, Sweden.
Curr Genet. 1999 Mar;35(2):82-7. doi: 10.1007/s002940050436.
We have investigated the effects of inhibition of histone de-acetylase activity on silencing at the silent mating-type loci in fission yeast. Treatment of exponentially growing cells with the histone deacetylase inhibitor, trichostatin A (TSA), resulted in derepression of a marker gene inserted 150 bp distal from the silent mat3-M locus. The natural targets for the silencing mechanism in this region were only partially derepressed and the activation appeared to be asymmetric, i.e. the mat2-P cassette remained silent at concentrations that clearly partially derepressed the mat3-M cassette. We further noted that treatment of wild-type h90 cells resulted in the generation of altered sporulation phenotypes, indicating that the treatment affected the expression of mating-type genes and/or mating-type switching. The results are discussed in the light of recent accumulated data regarding the role of deacetylation for silencing in other species.
我们研究了组蛋白去乙酰化酶活性抑制对裂殖酵母中沉默交配型位点沉默的影响。用组蛋白去乙酰化酶抑制剂曲古抑菌素A（TSA）处理指数生长的细胞，导致插入在沉默的mat3-M位点远端150 bp处的标记基因去抑制。该区域沉默机制的天然靶点仅部分去抑制，且激活似乎是不对称的，即mat2-P盒在明显部分去抑制mat3-M盒的浓度下仍保持沉默。我们还注意到，处理野生型h90细胞会导致产生改变的孢子形成表型，表明该处理影响了交配型基因的表达和/或交配型转换。根据最近积累的关于去乙酰化在其他物种沉默中的作用的数据对结果进行了讨论。
