Kim Y B, Honda A, Yoshida M, Horinouchi S
Department of Biotechnology, Graduate School of Agriculture and Life Sciences, The University of Tokyo, Japan.
FEBS Lett. 1998 Oct 2;436(2):193-6. doi: 10.1016/s0014-5793(98)01124-7.
A gene named phd1+ encoding a protein highly homologous to the yeast and human histone deacetylases, such as Saccharomyces cerevisiae Rpd3p and human HDAC1, was cloned from Schizosaccharomyces pombe. The immune complex isolated from S. pombe cells expressing Phd1 fused to the FLAG epitope showed histone deacetylase activity, which was inhibited by trichostatin A (TSA), a specific inhibitor of histone deacetylase. The null mutation of phd1+ resulted in a marked decrease in the total cellular histone deacetylase activity and an increase in the sensitivity to TSA. Although the phd1 disruptant showed no obvious defect in the mitotic cell cycle or mating, both homothallic haploid and heterothallic diploid cells failed to form spores in the absence of phd1+. These results indicate that phd1+ encodes a histone deacetylase, which is involved in the meiotic cell cycle in S. pombe.
从粟酒裂殖酵母中克隆出一个名为phd1+的基因，该基因编码一种与酵母和人类组蛋白去乙酰化酶高度同源的蛋白质，如酿酒酵母Rpd3p和人类HDAC1。从表达与FLAG表位融合的Phd1的粟酒裂殖酵母细胞中分离出的免疫复合物显示出组蛋白去乙酰化酶活性，该活性被组蛋白去乙酰化酶的特异性抑制剂曲古抑菌素A（TSA）抑制。phd1+的无效突变导致细胞总组蛋白去乙酰化酶活性显著降低，对TSA的敏感性增加。尽管phd1缺失突变体在有丝分裂细胞周期或交配方面没有明显缺陷，但同宗配合的单倍体细胞和异宗配合的二倍体细胞在没有phd1+的情况下都无法形成孢子。这些结果表明，phd1+编码一种组蛋白去乙酰化酶，它参与了粟酒裂殖酵母的减数分裂细胞周期。
