使用1.48微米二极管激光束提高牛体外成熟/体外受精/体外培养囊胚的孵化率
Enhanced hatching rate of bovine IVM/IVF/IVC blastocysts using a 1.48-micron diode laser beam.
作者信息
Park S, Kim E Y, Yoon S H, Chung K S, Lim J H
机构信息
Maria Infertility Medical Institute, Sinseol-Dong Dongdaemun-Gu, Seoul, Korea.
出版信息
J Assist Reprod Genet. 1999 Feb;16(2):97-101. doi: 10.1023/a:1022573024170.
PURPOSE
Our purpose was to test whether zona pellucida (ZP) drilling using a 1.48-micron diode laser beam on bovine IVM/IVF/IVC blastocysts is effective for embryo hatching.
METHODS
Blastocysts produced in vitro at day 7 after IVF were divided into control and laser-drilled groups, respectively.
RESULTS
When the rates of in vitro development of bovine embryos were examined, the average cleavage rate (> or = two-cell) was 82.3% and the blastocyst rate at day 7 after IVF was 32.5%. Using these blastocysts, when the laser drilling effect was investigated at 48 hr after treatment, the total hatching rate in the laser-drilled group (98.0%) was significantly higher than that in the control group (60.0%) (P < 0.001). Especially, the hatched rate of the laser-drilled group (68.0%) was significantly enhanced compared with that of the control group (30.0%) (P < 0.001).
CONCLUSIONS
These results demonstrated that laser ZP drilling on bovine IVM/IVF/IVC blastocysts can significantly increase the hatching rate.
目的
我们的目的是测试使用1.48微米二极管激光束对牛体外成熟/体外受精/体外培养囊胚进行透明带（ZP）打孔是否对胚胎孵化有效。
方法
体外受精后第7天体外产生的囊胚分别分为对照组和激光打孔组。
结果
检查牛胚胎的体外发育率时，平均分裂率（≥二细胞）为82.3%，体外受精后第7天的囊胚率为32.5%。使用这些囊胚，在处理后48小时研究激光打孔效果时，激光打孔组的总孵化率（98.0%）显著高于对照组（60.0%）（P<0.001）。特别是，激光打孔组的孵化率（68.0%）与对照组（30.0%）相比显著提高（P<0.001）。
结论
这些结果表明，对牛体外成熟/体外受精/体外培养囊胚进行激光透明带打孔可显著提高孵化率。
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