Laufer N, Palanker D, Shufaro Y, Safran A, Simon A, Lewis A
Department of Obstetrics and Gynecology, Hadassah University Hospital, Jerusalem, Israel.
Fertil Steril. 1993 Apr;59(4):889-95. doi: 10.1016/s0015-0282(16)55877-4.
To examine the efficiency of argon fluoride excimer laser drilling of the zona pellucida of mouse oocytes in improving in vitro fertilization (IVF) at low sperm concentrations and to assess its safety.
Oocytes obtained from (Balb/c x C57BL6)CB6F1 female mice were drilled by laser and divided into two groups: group I (89 oocytes) were inseminated with 10(5) sperm cells/mL, and group II (94 oocytes) were inseminated with 10(6) sperm cells/mL. Both groups' fertilization rate and development in vitro was compared with control oocytes that underwent the same preparation steps but no drilling (94 and 88 oocytes for group I and group II, respectively).
The fertilization rate and the development in vitro of the laser-drilled groups is compared with that of the control. In addition, in vivo development of embryos generated from laser-drilled oocytes after transfer to pseudopregnant recipients is assessed.
For both sperm concentrations, laser drilling significantly enhanced fertilization over control (67% versus 31% at 10(5) sperm cells/mL and 90% versus 54% at 10(6) sperm cells/mL). The development into the blastocyst stage after 96 hours of incubation was similar for both the laser-drilled and control groups at any sperm cell concentration. However, complete hatching at this point was significantly enhanced by the drilling procedure. Normal litters were obtained from the transfer of embryos developed from zona-drilled oocytes into pseudopregnant recipients.
Excimer laser drilling enhanced IVF at low sperm cell concentration. The procedure is safe and did not interfere with embryo development in vitro or in vivo.
研究氟化氩准分子激光打孔处理小鼠卵母细胞透明带在低精子浓度下对体外受精（IVF）的改善效果，并评估其安全性。
从（Balb/c×C57BL6）CB6F1雌性小鼠获取的卵母细胞经激光打孔后分为两组：第一组（89个卵母细胞）用10⁵个精子细胞/mL授精，第二组（94个卵母细胞）用10⁶个精子细胞/mL授精。将两组的受精率和体外发育情况与经过相同准备步骤但未打孔的对照卵母细胞（第一组和第二组分别为94个和88个卵母细胞）进行比较。
将激光打孔组的受精率和体外发育情况与对照组进行比较。此外，评估激光打孔卵母细胞产生的胚胎移植到假孕受体后的体内发育情况。
对于两种精子浓度，激光打孔组的受精率均显著高于对照组（10⁵个精子细胞/mL时为67%对31%，10⁶个精子细胞/mL时为90%对54%）。在任何精子细胞浓度下，激光打孔组和对照组在培养96小时后发育到囊胚阶段的情况相似。然而，此时的完全孵化通过打孔程序显著提高。将透明带打孔卵母细胞发育而来的胚胎移植到假孕受体后获得了正常产仔。
准分子激光打孔提高了低精子细胞浓度下的体外受精率。该程序是安全的，且不干扰胚胎的体外或体内发育。
