Malter H E, Cohen J
Department of Gynecology and Obstetrics, Emory University, Atlanta, Georgia.
Fertil Steril. 1989 Jan;51(1):139-48. doi: 10.1016/s0015-0282(16)60443-0.
Partial zona dissection (PZD), a method using mechanical force to open the human zona pellucida, and zona drilling, which uses acidic Tyrode's (AT) medium, were compared in 1-day-old oocytes prior to reinsemination. The incidences of monospermy and polyspermy were 13/54 (24%) and 14/54 (26%) following PZD and 6/46 (13%) and 8/46 (17%) following the use of AT medium. This compared favorably with conventional reinsemination: 15/161 (9%) monospermy and 4/161 (3%) polyspermy. Three of the 27 PZD embryos became blastocysts, while none of the AT-exposed embryos developed satisfactorily. Eleven male-factor couples had some of their oocytes randomly treated with PZD prior to insemination; each of the patients had non-micromanipulated control oocytes. Monospermic fertilization and cleavage (23/34; 68%) doubled (P less than 0.05) when PZD was compared with the control oocytes (10/30; 33%). Replacing two PZD and a single control embryo in two patients resulted in twin pregnancies. A third twin pregnancy was established following replacement of only micromanipulated embryos.
在再次受精前，对1日龄卵母细胞比较了部分透明带分离术（PZD，一种使用机械力打开人透明带的方法）和透明带钻孔术（使用酸性台氏液[AT]培养基）。PZD后单精受精和多精受精的发生率分别为13/54（24%）和14/54（26%），使用AT培养基后分别为6/46（13%）和8/46（17%）。这与传统的再次受精相比更具优势：单精受精15/161（9%），多精受精4/1,61（3%）。27个经PZD处理的胚胎中有3个发育成囊胚，而暴露于AT的胚胎均未令人满意地发育。11对男性因素导致不孕的夫妇，其部分卵母细胞在受精前随机接受PZD处理；每位患者都有未进行显微操作的对照卵母细胞。与对照卵母细胞（10/30；33%）相比，PZD时单精受精和卵裂（23/34；68%）增加了一倍（P<0.05）。在两名患者中植入两个经PZD处理的胚胎和一个对照胚胎后，成功实现了双胎妊娠。仅植入显微操作的胚胎后，又成功实现了一例双胎妊娠。
