Lubbe M M, Stanley K, Chalkley L J
Department of Medical Microbiology, Faculty of Health Sciences, University of the Orange Free State, Bloemfontein, South Africa.
FEMS Microbiol Lett. 1999 Mar 1;172(1):79-83. doi: 10.1111/j.1574-6968.1999.tb13453.x.
This study investigated the prevalence of nim genes (proposed to encode a 5-nitroimidazole resistance product) in 64 anaerobic/facultative anaerobic bacteria. Employing universal nim gene primers, 458-bp amplified fragments were recorded as presumptive positives in 22/64 strains at an annealing temperature of 52 degrees C and 15/64 strains at 62 degrees C, of which seven were propionibacteria. DNA sequencing confirmed the presence of nimA genes in Propionibacterium spp. (five strains), Actinomyces odontolyticus (one strain), Prevotella bivia (one strain) and Clostridium bifermentans (one strain) and nimB genes from five strains of Bacteroides fragilis. nimA genes were predominant in propionibacteria indicating a potential nimA gene source in anaerobic environments.
本研究调查了64株厌氧/兼性厌氧细菌中nim基因（推测编码5-硝基咪唑抗性产物）的流行情况。使用通用nim基因引物，在52℃退火温度下，22/64株菌株中记录到458bp的扩增片段为推定阳性，在62℃时为15/64株菌株，其中7株为丙酸杆菌。DNA测序证实丙酸杆菌属（5株）、溶牙放线菌（1株）、二路普雷沃菌（1株）和双发酵梭菌（1株）中存在nimA基因，以及来自5株脆弱拟杆菌的nimB基因。nimA基因在丙酸杆菌中占主导地位，表明在厌氧环境中存在潜在的nimA基因来源。
