Hardt J, Angerer J
Institute and Outpatient Clinic of Occupational, Social, and Environmental Medicine, University of Erlangen-Nürnberg, Erlangen, Germany.
J Chromatogr B Biomed Sci Appl. 1999 Feb 19;723(1-2):139-45. doi: 10.1016/s0378-4347(98)00477-0.
Human metabolism of the insecticide propoxur yields 2-isopropoxyphenol (IPP) which is excreted conjugated in urine. In this publication a sensitive and selective analytical method is described which permits the determination of IPP as a suitable parameter for biomonitoring. The clean-up of the hydrolysed urine samples consisted of steam distillation and solid-phase extraction using a reversed-phase column. IPP and the internal standard 2-ethoxyphenol were converted to their pentafluorobenzyl ethers. Excess of the derivatisation reagent was removed using deactivated silica gel. Separation and quantitative analysis was carried out by capillary gas chromatography and mass selective detection. Coefficients of variation were below 5% for concentrations from 6 to 300 microg/l. The detection limit was 0.5 microg/l. The method was checked by analysing six urine samples from pest controllers after indoor application of propoxur. The IPP concentrations ranged from 45 to 306 microg/g creatinine. IPP was not detected in urine specimens from 10 non-exposed persons. The sensitivity of the developed method permits the detection of latent exposure to propoxur.
杀虫剂残杀威在人体中的代谢产物为2-异丙氧基苯酚（IPP），它以结合物的形式经尿液排出。本出版物描述了一种灵敏且具选择性的分析方法，该方法可将IPP测定作为生物监测的一个合适参数。水解后的尿液样本净化过程包括水蒸气蒸馏和使用反相柱的固相萃取。IPP和内标物2-乙氧基苯酚被转化为它们的五氟苄基醚。使用失活硅胶去除过量的衍生化试剂。通过毛细管气相色谱和质量选择性检测进行分离和定量分析。浓度在6至300微克/升范围内时，变异系数低于5%。检测限为0.5微克/升。通过分析在室内施用残杀威后来自害虫防治人员的六个尿液样本对该方法进行了检验。IPP浓度范围为45至306微克/克肌酐。在10名未接触者的尿液样本中未检测到IPP。所开发方法的灵敏度能够检测出对残杀威的潜在接触。
