Leenheers L H, van Breugel D C, Ravensberg J C, Meuling W J, Jongen M J
Department of Occupational Toxicology, TNO Medical Biological Laboratory, Rijswijk, Netherlands.
J Chromatogr. 1992 Jul 24;578(2):189-94. doi: 10.1016/0378-4347(92)80415-m.
An analytical method for the assessment of the exposure of workers to the pesticide propoxur through biological monitoring has been developed. This study was part of a survey of occupational exposure to pesticides used in greenhouses for the growing of ornamental plants. In order to assess the actual absorbed amount of propoxur in the body, an analytical method for its metabolite 2-isopropoxyphenol in urine was required. This led to the development of a gas chromatographic-mass spectrometric assay involving hydrolysis and solvent extraction. A mass-selective detector, operated in single-ion mode, provides a selective and sensitive quantification of 2-isopropoxyphenol with a detection limit of 6 micrograms/l. The method has been validated with respect to the hydrolysis of urine samples, analytical recovery of 2-isopropoxyphenol, stability of its conjugates, limit of detection, background and precision. The analytical recovery from spiked urine was over 95%. 2-Isopropoxyphenol was excreted in urine as a conjugate and was stable for at least seven months when stored at -20 degrees C. It was not detected in urine from non-exposed persons. Between-day coefficients of variation were 20, 10, 7 and 4% for concentrations of 15, 29, 150 and 213 micrograms/l, respectively. Measured as 2-isopropoxyphenol, ca. 80% of an orally administered dose of propoxur was excreted in urine within 10 h.
已开发出一种通过生物监测评估工人接触农药残杀威情况的分析方法。本研究是对温室中用于种植观赏植物的农药职业接触情况调查的一部分。为了评估残杀威在体内的实际吸收量，需要一种分析尿液中其代谢物2 - 异丙氧基苯酚的方法。这促使开发了一种涉及水解和溶剂萃取的气相色谱 - 质谱分析法。以单离子模式运行的质量选择检测器能够对2 - 异丙氧基苯酚进行选择性和灵敏的定量分析，检测限为6微克/升。该方法在尿液样本的水解、2 - 异丙氧基苯酚的分析回收率、其结合物的稳定性、检测限、背景和精密度方面均已得到验证。加标尿液的分析回收率超过95%。2 - 异丙氧基苯酚以结合物形式随尿液排出，在 - 20℃储存时至少七个月保持稳定。在未接触者的尿液中未检测到该物质。对于浓度分别为15、29、150和213微克/升的情况，日间变异系数分别为20%、10%、7%和4%。以2 - 异丙氧基苯酚计，口服剂量的残杀威约80%在10小时内随尿液排出。
