Ide N, Hata Y, Deguchi M, Hirao K, Yao I, Takai Y
Takai Biotimer Project, ERATO, Japan Science and Technology Corporation, c/o JCR Pharmaceuticals Co. Ltd., Kobe, 651-2241, Japan.
Biochem Biophys Res Commun. 1999 Mar 24;256(3):456-61. doi: 10.1006/bbrc.1999.0364.
Synaptic scaffolding molecule (S-SCAM) is a multiple PDZ domain-containing protein, which interacts with neuroligin, a cell adhesion molecule, and the NMDA receptor. In this study, we searched for S-SCAM-interacting proteins and obtained a neuralplakophilin-related armadillo-repeat protein (NPRAP)/delta-catenin. NPRAP/delta-catenin bound to the last PDZ domain of S-SCAM via its carboxyl-terminus in three different cell-free assay systems, was coimmunoprecipitated with S-SCAM from rat crude synaptosomes, and was localized at the excitatory synapses in rat hippocampal neurons. NPRAP/delta-catenin may be implicated in the molecular organization of synaptic junctions through the interaction with S-SCAM.
突触支架分子（S-SCAM）是一种含有多个PDZ结构域的蛋白质，它与细胞粘附分子神经连接蛋白以及NMDA受体相互作用。在本研究中，我们寻找与S-SCAM相互作用的蛋白质，并获得了一种神经plakophilin相关的犰狳重复蛋白（NPRAP）/δ-连环蛋白。在三种不同的无细胞检测系统中，NPRAP/δ-连环蛋白通过其羧基末端与S-SCAM的最后一个PDZ结构域结合，在大鼠粗制突触小体中与S-SCAM进行了共免疫沉淀，并定位在大鼠海马神经元的兴奋性突触处。NPRAP/δ-连环蛋白可能通过与S-SCAM的相互作用参与突触连接的分子组织。
