Shimozato O, Takeda K, Yagita H, Okumura K
Department of Immunology, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan.
Biochem Biophys Res Commun. 1999 Mar 24;256(3):519-26. doi: 10.1006/bbrc.1999.0336.
CD30, a member of the TNF receptor family, has been implicated in the activation of T cells and B cells. In the present study, we characterized the expression and function of murine CD30 ligand (mCD153) by utilizing mCD153 transfectants and a novel mAb against mCD153 (RM153), which can inhibit the binding of murine CD30 to mCD153. The mCD153 transfectants did not co-stimulate the proliferation of anti-CD3-stimulated naive T cells but enhanced the proliferation of anti-CD28-co-stimulated T cells. The mCD153 transfectants exhibited a potent co-stimulatory activity for proliferation of pre-activated T cells that expressed CD30 after anti-CD3 and anti-CD28 stimulation. In contrast to the CD30 expression on naive T cells that required anti-CD28 co-stimulation, mCD153 expression was observed on anti-CD3-stimulated T cells without the anti-CD28 co-stimulation, predominantly on CD4(+) T cells with a transient kinetics which peaked at 24 h but disappeared at 48 h. In contrast to the preferential expression of CD30 on Th2 cells, mCD153 was expressed on both Th1 and Th2 cells after anti-CD3 stimulation. These results indicated a differential regulation of CD30 and CD153 expression in T cells, which may be relevant to immuno-regulatory role of the CD30-CD153 interaction.
CD30是肿瘤坏死因子受体家族的一员，与T细胞和B细胞的激活有关。在本研究中，我们通过利用mCD153转染子和一种针对mCD153的新型单克隆抗体（RM153）来抑制小鼠CD30与mCD153的结合，从而对小鼠CD30配体（mCD153）的表达和功能进行了表征。mCD153转染子不能共刺激抗CD3刺激的未活化T细胞的增殖，但能增强抗CD28共刺激的T细胞的增殖。mCD153转染子对经抗CD3和抗CD28刺激后表达CD30的预活化T细胞的增殖表现出强大的共刺激活性。与未活化T细胞上需要抗CD28共刺激才能表达CD30不同，在没有抗CD28共刺激的情况下，抗CD3刺激的T细胞上可观察到mCD153的表达，主要在CD4(+) T细胞上，其动力学呈短暂性，在24小时达到峰值，但在48小时消失。与CD30在Th2细胞上的优先表达不同，抗CD3刺激后，mCD153在Th1和Th2细胞上均有表达。这些结果表明T细胞中CD30和CD153表达的差异调节，这可能与CD30-CD153相互作用在免疫调节中的作用有关。
