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[公牛精子中DNA数量的定位与评估方法]

[Method of localization and evaluation of DNA quantities in bull spermatozoa].

作者信息

Henriet L

出版信息

Andrologia. 1976;8(4):326-39.

PMID:1008264
Abstract

The author attempted to evaluate the DNA quantities by means of a spectrophotometric method of UV light absorption. In a first publication data have been reported on the RNA quantities obtained by this method. This one concerns the data obtained on DNA. By this method, the manipulations on the spermatozoon are more dimple and sources of error are so discarded. It was so possible to get individual data on each spermatozoon, what is possible with chimical methods which give global quantities for a whole sample of semen. It is moreover possible to delimite the localization of DNA and to measure the amounts in each localization. The scale of the obtained data is large enough for information to several workers. The founded quantities and variations in data will be discussed in a following paper.

摘要

作者试图通过紫外光吸收分光光度法评估DNA含量。在第一篇出版物中,已经报道了用这种方法获得的RNA含量数据。这一篇涉及通过该方法获得的DNA数据。通过这种方法,对精子的操作更简单,并且消除了误差来源。因此有可能获得每个精子的个体数据,而化学方法只能给出整个精液样本的总量,无法做到这一点。此外,还可以确定DNA的定位并测量每个定位中的含量。所获得的数据范围足够大,可供多位研究人员参考。所测定的含量和数据变化将在后续论文中进行讨论。

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