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[Ultraspectrophotometric method of differential determination of DNA and RNA in spermatozoa].

作者信息

Henriet L, Berthet P, Lehoucq J C

出版信息

Andrologia. 1977 Jul-Sep;9(3):279-88.

PMID:907213
Abstract

The authors describe a complement of the previously published ultramicrospectro-photometric method for the dosage of nucleic acids. The spermatozoa are scanned by a bundle of U-V. light. It is so possible to obtain the measurement of the U-V. light absorption on a very big number of very small areas. The data are printed very accurately on one histogram. Thereafter spermatozoa are submitted to the action of RNase and DNase and rescanned with U-V. light. The comparison of the histograms allows to estimate the amount of nucleic acids. Further investigations are requested.

摘要

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