Seiki T, Oka S, Terayama K, Imiya K, Kawasaki T
Department of Biological Chemistry and CREST (Core Research for Educational Science and Technology) Project, Japan Science and Technology Corporation, Kyoto, 606-8501, Japan.
Biochem Biophys Res Commun. 1999 Feb 5;255(1):182-7. doi: 10.1006/bbrc.1999.0151.
A cDNA encoding a novel glucuronyltransferase was cloned from a rat brain cDNA library. The cDNA sequence contained an open reading frame encoding 324 amino acids, with type II transmembrane topology. The amino acid sequence revealed 49% homology to rat GlcAT-P, a glucuronyltransferase involved in the biosynthesis of the HNK-1 carbohydrate epitope of glycoproteins, [Terayama et al. (1997) Proc. Natl. Acad. Sci. USA 94, 6093-6098] and the highest sequence homology was found in the catalytic region. Northern blot analysis indicated that this newly cloned glucuronyltransferase is expressed in the nervous system, consistent with the selective localization of the HNK-1 carbohydrate epitope in the nervous system. Transfection of this cDNA into COS-1 cells induced the expression of the HNK-1 carbohydrate epitope on cell surfaces, and induced the morphological changes in these cells. These results indicated that this newly cloned cDNA is a second glucuronyltransferase involved in the biosynthesis of the HNK-1 carbohydrate epitope.
从大鼠脑cDNA文库中克隆出一个编码新型葡萄糖醛酸转移酶的cDNA。该cDNA序列包含一个编码324个氨基酸的开放阅读框，具有II型跨膜拓扑结构。氨基酸序列显示与大鼠GlcAT-P有49%的同源性，GlcAT-P是一种参与糖蛋白HNK-1碳水化合物表位生物合成的葡萄糖醛酸转移酶，[寺山等人（1997年）《美国国家科学院院刊》94，6093 - 6098]，且在催化区域发现了最高的序列同源性。Northern印迹分析表明，这种新克隆的葡萄糖醛酸转移酶在神经系统中表达，这与HNK-1碳水化合物表位在神经系统中的选择性定位一致。将该cDNA转染到COS-1细胞中可诱导HNK-1碳水化合物表位在细胞表面表达，并诱导这些细胞发生形态变化。这些结果表明，这个新克隆的cDNA是参与HNK-1碳水化合物表位生物合成的第二种葡萄糖醛酸转移酶。
