Thiery I, Hamon S
Unité des Bactéries Entomopathogènes, Institut Pasteur, Paris, France.
J Am Mosq Control Assoc. 1998 Dec;14(4):472-6.
Bacillus thuringiensis var. israelensis and Bacillus sphaericus products were assayed against their respective reference powders IPS82 and SPH88. Since their production in 1982 and 1988, the potency and larvicidal activity of these standard powders have been regularly checked on their test insects Aedes aegypti (for IPS82) or Culex pipiens (for SPH88). Over the 16-year evaluation period of IPS82 and 10-year evaluation period of SPH88, their potencies were considered stable. The global mean of each year's mean showed a coefficient of variation of less than 20%. Larval rearing was the most important factor in the reproducibility of the bioassay, although some variation also originated from the person performing the bioassay. This study demonstrated that the SPH88 standard could be kept in a stock suspension at 4 degrees C for 3 years without loss of potency. Moreover, after 9 years of storage in suspension, only a 2-fold decrease in the potency of SPH88 was detected.
苏云金芽孢杆菌以色列变种和球形芽孢杆菌产品针对其各自的参考粉剂IPS82和SPH88进行了测定。自1982年和1988年生产以来，这些标准粉剂的效力和杀幼虫活性已定期在其测试昆虫埃及伊蚊（针对IPS82）或致倦库蚊（针对SPH88）上进行检查。在IPS82的16年评估期和SPH88的10年评估期内，它们的效力被认为是稳定的。每年平均值的全球均值显示变异系数小于20%。幼虫饲养是生物测定重现性中最重要的因素，尽管有些变异也源于进行生物测定的人员。本研究表明，SPH88标准品可以在4℃的储备悬液中保存3年而不丧失效力。此外，在悬液中储存9年后，仅检测到SPH88的效力下降了2倍。
