Lacey L A, Lacey C M, Padua L E
Insects Affecting Man and Animals Research Lab, USDA, ARS, Gainesville, FL 32604.
J Am Mosq Control Assoc. 1988 Mar;4(1):39-43.
Laboratory bioassay of the PG-14 isolate of Bacillus thuringiensis var. morrisoni (serotype 8a:8b) against early fourth instar larvae of 8 species of mosquitoes revealed a range of susceptibilities similar to the susceptibilities of these species to Bacillus thuringiensis var. israelensis (serotype 14). The most susceptible species were: Culex quinquefasciatus, Cx. salinarius, Anopheles albimanus and Aedes aegypti. The least susceptible species tested was An. quadrimaculatus. Separate bioassays of PG-14 against the four instars of Ae. aegypti demonstrated a strong negative correlation (R = -0.97) between larval age and susceptibility. Temperature significantly affected the stability of larvicidal toxin in aqueous suspensions of PG-14. Larvicidal activity of a bacterial suspension was nearly completely eliminated after 132 days of storage at 31 degrees C, but was essentially unchanged for those suspensions stored at 4 degrees C.
苏云金芽孢杆菌莫里森变种（血清型8a:8b）PG-14分离株针对8种蚊虫四龄初期幼虫的实验室生物测定显示，其敏感性范围与这些物种对苏云金芽孢杆菌以色列变种（血清型14）的敏感性相似。最敏感的物种为：致倦库蚊、盐泽库蚊、白纹伊蚊和埃及伊蚊。测试中最不敏感的物种是四斑按蚊。PG-14针对埃及伊蚊四个龄期的单独生物测定表明，幼虫年龄与敏感性之间存在很强的负相关（R = -0.97）。温度显著影响PG-14水悬浮液中杀幼虫毒素的稳定性。细菌悬浮液在31摄氏度储存132天后，杀幼虫活性几乎完全丧失，但在4摄氏度储存的悬浮液基本没有变化。
