Kaneko M, Kawakita T, Nomoto K
Kampo (Traditional Chinese Medicine) Research Laboratories, Kanebo Co., Ltd., Osaka, Japan.
Immunopharmacol Immunotoxicol. 1999 Feb;21(1):125-40. doi: 10.3109/08923979909016398.
Our previous study showed that the serum level of antigen-specific IgE antibodies in primary response was decreased by a traditional Chinese medicine, Bu-zhong-yi-qi-tang (Japanese name; Hochu-ekki-to, HOT). In this study, we examined inhibition of secondary IgE response and of eosinophil infiltration by HOT. BALB/c mice were intraperitoneally immunized with aluminum hydroxide adsorbed with DNP-KLH (DNP-KLH + alum) on day -14 and on day 0. In mice treated with HOT daily from day -14, the serum level of antigen-specific IgE antibodies after the secondary immunization was significantly decreased compared to that in mice not treated with HOT. Eosinophils increased in number after 6 and 24 hr, and CD4+ T cells in the peritoneal cavity increased in number 24 hr after the secondary immunization. HOT suppressed accumulation of eosinophils and CD4+ T cells in the peritoneal cavity. Furthermore, HOT suppressed the numbers of IL-4- and IL-5-producing cells 24 hr after the secondary immunization, but did not inhibit the number of IFN-gamma-producing cells. HOT also suppressed IL-5 mRNA expression. Furthermore, HOT also inhibited antigen-induced late-phase reaction (LPR) in the skin. These results suggested that HOT exhibited anti-allergic effects mainly by inhibiting Th2 cell responses.
我们之前的研究表明，中药补中益气汤（日本名称：Hochu-ekki-to，HOT）可降低初次免疫应答中抗原特异性IgE抗体的血清水平。在本研究中，我们检测了HOT对再次IgE应答和嗜酸性粒细胞浸润的抑制作用。在第-14天和第0天，用吸附有二硝基苯基钥孔戚血蓝蛋白（DNP-KLH）的氢氧化铝（DNP-KLH+明矾）对BALB/c小鼠进行腹腔免疫。从第-14天开始每天用HOT处理的小鼠，再次免疫后抗原特异性IgE抗体的血清水平与未用HOT处理的小鼠相比显著降低。再次免疫后6小时和24小时嗜酸性粒细胞数量增加，腹腔内CD4+T细胞数量在24小时增加。HOT抑制腹腔内嗜酸性粒细胞和CD4+T细胞的积聚。此外，HOT在再次免疫后24小时抑制产生IL-4和IL-5的细胞数量，但不抑制产生IFN-γ的细胞数量。HOT还抑制IL-5 mRNA表达。此外，HOT还抑制皮肤中抗原诱导的迟发性反应（LPR）。这些结果表明，HOT主要通过抑制Th2细胞应答发挥抗过敏作用。
