Kaneko M, Kishihara K, Kawakita T, Nakamura T, Takimoto H, Nomoto K
Kampo Research Laboratories, Kanebo Co. Ltd., Osaka, Japan.
Immunopharmacology. 1997 Apr;36(1):79-85. doi: 10.1016/s0162-3109(96)00162-2.
The ability of a traditional herbal medicine, Bu-zhong-yi-qi-tang (Japanese name: Hochu-ekki-to, HOT), to suppress IgE production was investigated. BALB/c mice were intraperitoneally immunized with aluminium hydroxide adsorbed with DNP-KLH (DNP-KLH + alum). When oral administration of HOT was begun just after immunization, the serum level of antigen-specific IgE was significantly decreased, although those of antigen-specific IgG1 and IgG2a were not influenced. In the culture of spleen cells obtained 14 days after immunization with DNP-KLH, antigen-specific IgE and IgG1 production by the cells of the HOT-treated mice was significantly suppressed compared to that in immunized mice. Furthermore, in the combination culture with CD4+ T cells and B cells separated from spleen cells, IgE production by the cells from immunized mice was inhibited by replacement of their corresponding cell population with either CD4+ T cells or B cells of HOT-treated mice. Additionally, production of interleukin 2 (IL-2) and IL-4 was significantly suppressed in HOT-treated mice but not that of IFN-gamma in comparison to the immunized mice. These results suggested that HOT decreased the IgE level in serum by inhibiting the development of IL-4-producing CD4+ T cells.
研究了传统草药补中益气汤（日语名称：Hochu-ekki-to，HOT）抑制IgE产生的能力。用吸附有DNP-KLH（DNP-KLH + 明矾）的氢氧化铝对BALB/c小鼠进行腹腔免疫。免疫后立即开始口服HOT，抗原特异性IgE的血清水平显著降低，而抗原特异性IgG1和IgG2a的血清水平不受影响。在用DNP-KLH免疫14天后获得的脾细胞培养物中，与免疫小鼠相比，HOT处理小鼠的细胞产生的抗原特异性IgE和IgG1显著受到抑制。此外，在与从脾细胞中分离出的CD4+ T细胞和B细胞的联合培养中，用HOT处理小鼠的CD4+ T细胞或B细胞替代免疫小鼠的相应细胞群体，可抑制免疫小鼠细胞产生IgE。另外，与免疫小鼠相比，HOT处理小鼠中白细胞介素2（IL-2）和IL-4的产生显著受到抑制，但干扰素-γ的产生不受影响。这些结果表明，HOT通过抑制产生IL-4的CD4+ T细胞的发育来降低血清中的IgE水平。
