Koenig H M
Ann Clin Res. 1976;8 Suppl 17:151-5.
Microcytic red blood cells (RBC) occur in iron-deficiency anemia, lead poisoning, and the thalassemia syndromes. Micromeasurement of FEP by acid extraction from RBC was performed on RBC of 64 subjects with RBC mean corpuscular volume less than 78 fl as determined on a Coulter S. FEP was also determined on RBC from 25 nonanemic, normocytic subjects for comparison. The 25 nonanemic subjects, 29 subjects with alpha-thalassemia trait and 16 subjects with beta-thalassemia trait had FEP less than 107 mugm/100 ml RBC. Nineteen microcytic subjects with iron-deficiency anemia had FEP of 185--752 mugm/100 ml RBC. Hemolysates from 8 lead intoxication individuals had FEP values similar to those of iron-deficient patients. The fluorescence emission spectra of lysates with high FEP, which were not extracted, were similar in iron deficiency and lead poisoning. The porphyrin that accumulates in these two conditions appears to be zinc protoporphyrin. Micromeasurement of FEP can be used to initially classify microcytic anemias into either a disturbance of globin synthesis or a disturbance in heme synthesis. Iron-deficiency anemia and lead poisoning cause accumulation of identical prophyrin and cannot be distinguished by fluorometric analysis.
小红细胞见于缺铁性贫血、铅中毒和地中海贫血综合征。对64名红细胞平均容积小于78飞升（根据库尔特S型血细胞计数器测定）的受试者的红细胞进行了酸提取法微量测定游离原卟啉（FEP）。还对25名非贫血、正常红细胞的受试者的红细胞进行了FEP测定以作比较。25名非贫血受试者、29名具有α地中海贫血特征的受试者和16名具有β地中海贫血特征的受试者的FEP小于107微克/100毫升红细胞。19名缺铁性贫血的小红细胞受试者的FEP为185 - 752微克/100毫升红细胞。8名铅中毒个体的溶血产物的FEP值与缺铁患者的相似。未提取的高FEP溶血产物的荧光发射光谱在缺铁和铅中毒时相似。在这两种情况下积累的卟啉似乎是锌原卟啉。FEP的微量测定可用于初步将小红细胞贫血分为珠蛋白合成障碍或血红素合成障碍。缺铁性贫血和铅中毒会导致相同卟啉的积累，无法通过荧光分析加以区分。
