Methner U, Barrow P A
Bundesinstitut für gesundheitlichen Verbraucherschutz und Veterinärmedizin, Fachbereich Jena.
Berl Munch Tierarztl Wochenschr. 1997 Oct;110(10):391-6.
Methods of immunoprophylaxis for poultry using live Salmonella vaccines are increasingly gaining in importance. Methods of a simple and reliable bacteriological as well as serological differentiation between vaccine and field strains will be of decisive importance for the acceptance and use of live Salmonella vaccines. The absence of motility in Salmonella strains may be a marker fulfilling these criteria. The studies described served to examine whether virulence and the ability to inhibit other Salmonella strains could be influenced by the absence of motility in Salmonella (S.) Enteritidis and (S.) Typhimurium. In a cell-culture model (IEC 6) under in vitro conditions, non-motile transposon mutants (TnphoA) of S. Enteritidis and S. Typhimurium exhibited a clearly reduced invasion potential in comparison with the respective motile parental strain. Under in vitro conditions (nutrient broth culture), the inhibitory potential of these non-motile mutants was also reduced compared to the motile original strains. In contrast, in vivo studies in a-few-days-old chickens revealed that there was no reduction of the virulence of non-motile mutants of S. Enteritidis and S. Typhimurium in comparison with the motile parental strain. In day-old chicks, the inhibitory potential of non-motile strains was significantly reduced and in some cases, had even become completely lost.
使用活沙门氏菌疫苗对家禽进行免疫预防的方法正变得越来越重要。对于活沙门氏菌疫苗的接受和使用而言，能够简单可靠地区分疫苗株和野毒株的细菌学及血清学方法将具有决定性意义。沙门氏菌菌株缺乏运动性可能是满足这些标准的一个标志。所描述的研究旨在检验肠炎沙门氏菌和鼠伤寒沙门氏菌缺乏运动性是否会影响其毒力以及抑制其他沙门氏菌菌株的能力。在体外条件下的细胞培养模型（IEC 6）中，肠炎沙门氏菌和鼠伤寒沙门氏菌的非运动性转座子突变体（TnphoA）与各自的运动性亲本菌株相比，侵袭潜力明显降低。在体外条件下（营养肉汤培养），这些非运动性突变体的抑制潜力与运动性原始菌株相比也有所降低。相比之下，在几日龄雏鸡中的体内研究表明，肠炎沙门氏菌和鼠伤寒沙门氏菌非运动性突变体的毒力与运动性亲本菌株相比并未降低。在一日龄雏鸡中，非运动性菌株的抑制潜力显著降低，在某些情况下甚至完全丧失。
