Porter S B, Curtiss R
Department of Biology, Washington University, St. Louis, Missouri 63130, USA.
Avian Dis. 1997 Jan-Mar;41(1):45-57.
Invasion of Salmonella into the cells of the intestinal epithelium is an important step in the infection process. This initial invasion is followed by colonization of other organs throughout the body. In an attempt to better understand this process, we moved defined mutations in several genes of the inv locus into Salmonella typhimurium UK-1 and two strains of Salmonella enteritidis. These mutant strains were evaluated for their oral and intraperitoneal virulence as determined by 50% lethal dose in 1-day-old white leghorn chicks. These inv mutants were also studied for their ability to colonize orally infected chicks. The invA, invB, and invC mutations all caused a reduction in oral virulence and colonization by UK-1 and the S. enteritidis strains. Mutation of the invH gene had little or no effect on oral virulence or colonization. None of the inv genes tested had any effect on virulence of these Salmonella strains when administered intraperitoneally.
沙门氏菌侵入肠道上皮细胞是感染过程中的重要一步。这一初始侵入之后是在全身其他器官的定植。为了更好地理解这一过程，我们将inv位点几个基因中的特定突变导入鼠伤寒沙门氏菌UK-1和两株肠炎沙门氏菌。通过测定1日龄白来航鸡的50%致死剂量，评估这些突变菌株的口服和腹腔毒力。还研究了这些inv突变体在口服感染雏鸡中的定植能力。invA、invB和invC突变均导致UK-1和肠炎沙门氏菌菌株的口服毒力和定植能力降低。invH基因突变对口服毒力或定植几乎没有影响。当通过腹腔注射给药时，所测试的任何inv基因对这些沙门氏菌菌株的毒力均无影响。
