McCracken S A, Grant K E, MacKenzie I Z, Redman C W, Mardon H J
Nuffield Department of Obstetrics and Gynaecology, University of Oxford, The Women's Centre, John Radcliffe Hospital, Headington, Oxford, OX3 9DU, United Kingdom.
Biol Reprod. 1999 Apr;60(4):790-6. doi: 10.1095/biolreprod60.4.790.
A number of cytokines and their receptors are abundantly expressed at the materno-fetal interface and are thought to have a function in the regulation of placentation. Granulocyte-colony stimulating factor (G-CSF) is expressed by stromal cells in both placental tissue and maternal decidua throughout placentation. In this study, we examined the expression of placental G-CSF receptor (G-CSFR) mRNA and protein throughout gestation by ribonuclease protection assays, Western blotting, and immunohistochemistry. The major placental form of G-CSFR mRNA, corresponding to a membrane-bound form of the protein, was present in first-trimester placental tissues; levels decreased in second- and were highest in third-trimester placental tissues. Two placental G-CSFR molecules, 120 kDa and 150 kDa, were detected in first- and third-, but not second-, trimester tissues. The level of the 150-kDa G-CSFR was greater in the third- than in first-trimester samples. These differences were irrespective of whether or not the patients had received prostaglandin E1 analogues, prostaglandin E1 analogues and oxytocin, oxytocin alone, or mifepristone before labor. We demonstrated by immunohistochemistry that interstitial cytotrophoblast in first- and second-trimester decidual tissue and cytotrophoblast in term fetal membranes express G-CSFR. These data demonstrate that the expression of specific forms of placental G-CSFR is strictly cell type- and developmental stage-specific, and they suggest that G-CSFR may be important in decidual invasion of cytotrophoblast and in trophoblast function during placentation.
许多细胞因子及其受体在母胎界面大量表达，被认为在胎盘形成的调节中发挥作用。在整个胎盘形成过程中，胎盘组织和母体蜕膜中的基质细胞均表达粒细胞集落刺激因子（G-CSF）。在本研究中，我们通过核糖核酸酶保护分析、蛋白质印迹法和免疫组织化学，检测了整个妊娠期胎盘G-CSF受体（G-CSFR）mRNA和蛋白的表达。G-CSFR mRNA的主要胎盘形式，对应于该蛋白的膜结合形式，存在于孕早期胎盘组织中；其水平在孕中期降低，在孕晚期胎盘组织中最高。在孕早期和孕晚期组织中检测到两种胎盘G-CSFR分子，分别为120 kDa和150 kDa，但在孕中期组织中未检测到。150-kDa G-CSFR在孕晚期样本中的水平高于孕早期。这些差异与患者在分娩前是否接受过前列腺素E1类似物、前列腺素E1类似物和催产素、单独使用催产素或米非司酮无关。我们通过免疫组织化学证明，孕早期和孕中期蜕膜组织中的间质细胞滋养层以及足月胎膜中的细胞滋养层表达G-CSFR。这些数据表明，胎盘G-CSFR特定形式的表达具有严格的细胞类型和发育阶段特异性，提示G-CSFR可能在细胞滋养层的蜕膜侵袭和胎盘形成过程中的滋养层功能中起重要作用。
