胎儿体内的雌二醇受体（作者译）

[Oestradiol receptors in foetal compartment (author's transl)].

作者信息

Pasqualini J R, Sumida C, Nguyen B L, Gelly C

出版信息

Ann Endocrinol (Paris). 1976 Mar-Apr;37(2):89-90.

Abstract

Oestradiol concentration in the foetal plasma of guinea pig is 160-200 pg/ml (20-35 days of gestation), 200-300 pg/ml (35-45 days of gestation) and 170-450 pg/ml (45-60 days of gestation). After 3H-oestradiol administration to the foetus, this hormone circulates mainly as non-transformed 3H-oestradiol, 3H-oestrone and 3H-oestrone sulphate. 97-99% of the circulating oestradiol is found in unbound form. In vivo and in vitro studies in the foetus of guinea pig (40-55 days of gestation) show the presence of specific oestradiol receptors in the kidney, brain and Mullerian ducts of the foetus. The affinity constant in the cytosol of the brain is Kd=2 X 10(=9)M; in the cytosol kidney this value is Kd = 2.3 X 10(=10)M. A direct binding of 3H-oestradiol to the chromatin of the kidney was demonstrated with a Kd = 3.3 X 10(=10)M In the cytosol of Mullerian ducts the presence of a specific 3H-oestradiol macromolecule component with a sedimentation coefficient (in sucrose gradient) of 8-8.5S was detected. It is concluded that specific oestradiol receptors are present during foetal development in tissues such as kidney, brain and Mullerian ducts, but not in the liver.

摘要

豚鼠胎儿血浆中的雌二醇浓度在妊娠20 - 35天为160 - 200 pg/ml，妊娠35 - 45天为200 - 300 pg/ml，妊娠45 - 60天为170 - 450 pg/ml。给胎儿注射³H-雌二醇后，这种激素主要以未转化的³H-雌二醇、³H-雌酮和³H-雌酮硫酸盐的形式循环。循环中的雌二醇97 - 99%以未结合形式存在。对妊娠40 - 55天的豚鼠胎儿进行的体内和体外研究表明，胎儿的肾脏、大脑和苗勒管中存在特异性雌二醇受体。大脑胞质中的亲和常数Kd = 2×10⁻⁹M；肾脏胞质中的该值为Kd = 2.3×10⁻¹⁰M。³H-雌二醇与肾脏染色质的直接结合得到证实，Kd = 3.3×10⁻¹⁰M。在苗勒管的胞质中检测到一种特异性³H-雌二醇大分子成分，其沉降系数（在蔗糖梯度中）为8 - 8.5S。结论是，在胎儿发育过程中，肾脏、大脑和苗勒管等组织中存在特异性雌二醇受体，但肝脏中不存在。