Teng C S, Teng C T
Biochem J. 1976 Jan 15;154(1):1-9. doi: 10.1042/bj1540001.
After oestradiol administration in vivo, 87-95% of the initial concentration of oestradiol receptor in the cytoplasm of the embryonic-chick Müllerian-duct cell was translocated into the nucleus. The process of translocation depends on the amount of oestardiol administered in vivo. At 6 h after oestradiol administration in vivo, about 30% replenishment of the initial content of the cytosol receptor was observed in the cytoplasm. The Müllerian-duct nuclei, after exposure to non-radioactive oestradiol, exhibit saturable exchange with [3H]oestradiol in vitro. The exchange of oestradiol is temperature- and time-dependent. The optimal temperature and time for exchange are 37-41 degrees C and 2h respectively. The [3H]oestradiol-receptor complex extracted from the exchanged nuclei is present in 5-6S form, and its isoelectric point is 6.8. The number of nuclear oestradiol-binding sites of the developing Müllerian duct are 1.66, 2.22, 2.63, and 2.50 pmol/mg of DNA respectively for embryos of 10, 12, 15 and 18 days. The dissociation constants of the nuclear oestradiol receptor of the four observed developmental stages range from 3.0 to 3.1 nM.
在体内给予雌二醇后，胚胎期鸡苗勒氏管细胞胞质中初始浓度的雌二醇受体有87% - 95%转移到细胞核中。转移过程取决于体内给予的雌二醇量。在体内给予雌二醇6小时后，在胞质中观察到胞质溶胶受体初始含量约30%的补充。苗勒氏管细胞核在暴露于非放射性雌二醇后，在体外与[3H]雌二醇表现出可饱和交换。雌二醇的交换是温度和时间依赖性的。交换的最佳温度和时间分别为37 - 41摄氏度和2小时。从交换后的细胞核中提取的[3H]雌二醇 - 受体复合物以5 - 6S形式存在，其等电点为6.8。对于10、12、15和18天的胚胎，发育中的苗勒氏管的核雌二醇结合位点数量分别为1.66、2.22、2.63和2.50 pmol/mg DNA。四个观察到的发育阶段的核雌二醇受体的解离常数范围为3.0至3.1 nM。
