Jordan W P
Arch Dermatol. 1976 Dec;112(12):1741-4.
A direct assay using the mixed leukocyte migration inhibition technique for leukocyte inhibition factor (LIF) production has shown that nickel-sensitive subjects can be distinguished from nonallergic controls. The difference between the two groups' migrating indexes was obtained with nickel sulfate in 199 medium at a concentration of 150 mug/ml and without prior coupling to a carrier protein. A concise review of the principal in vitro assays for cell-mediated immunity is included.