Abai A M, Fekete B
Haematologia (Budap). 1982;15(1):111-25.
The agarose microdroplet leukocyte migration inhibition assay has been applied to the management of patients with lung cancer. Sera of patients with various stages of the disease were assayed in the indirect LMI system for their influence on the LIF production of normal leukocytes. A probably cell-mediated immunity blocking activity could be detected both in the serum of patients with inoperable tumours and in that of operable patients before and two weeks after surgery. The activity, however, disappeared in two thirds of the cases in about 6 months after surgery. The immune competence of patients with inoperable tumours was investigated before and during combined chemotherapy. A positive correlation was established between the tuberculin skin reactions and the leukocyte migration inhibition obtained in the direct LMI assay against PPD, but only in the presence of autologous serum. Sera of patients were also tested for putative in vivo LIF activity by their effect on the migration of autologous leukocytes. A significant correlation between anergy to PHA, as evaluated by skin testing, and the migration inhibition caused by sera was found. When, however, sera from healthy persons and from patients with chronic bronchitis or lung cancer were compared for their effect on the migration of normal leukocytes, no difference could be detected among the three groups. Single doses of the drugs temporarily weakened the skin reactions to PHA and the migration inhibition to PPD in autologous serum, but activity was restored when the drug influence had ceased.
琼脂糖微滴白细胞迁移抑制试验已应用于肺癌患者的治疗。在间接白细胞迁移抑制（LMI）系统中检测了不同疾病阶段患者的血清对正常白细胞产生白细胞抑制因子（LIF）的影响。在无法手术的肿瘤患者血清以及可手术患者术前和术后两周的血清中均检测到了可能的细胞介导免疫阻断活性。然而，这种活性在术后约6个月时在三分之二的病例中消失。对无法手术的肿瘤患者在联合化疗前及化疗期间的免疫能力进行了研究。在结核菌素皮肤反应与针对结核菌素纯蛋白衍生物（PPD）的直接LMI试验中获得的白细胞迁移抑制之间建立了正相关，但仅在存在自体血清的情况下。还通过患者血清对自体白细胞迁移的影响来检测其体内假定的LIF活性。发现通过皮肤试验评估的对PHA无反应性与血清引起的迁移抑制之间存在显著相关性。然而，当比较健康人以及慢性支气管炎或肺癌患者的血清对正常白细胞迁移的影响时，三组之间未检测到差异。单剂量药物暂时减弱了对PHA的皮肤反应以及自体血清中对PPD的迁移抑制，但当药物影响停止时活性恢复。
