Champion M J, Whitt G S
Biochem Genet. 1976 Oct;14(9-10):723-37. doi: 10.1007/BF00485337.
Allelic isozymes of glucosephosphate isomerase at the Gpi-A and -B loci were separated by starch gel electrophoresis in the warmouth (Lepomis gulosus) and green sunfish (L. cyanellus). The specific tissue distributions and developmental expressions of the GPI-A2, -AB, and -B2 isozymes were not different between these two species. The synchrony of allelic expression in normal intraspecific sunfish crosses was demonstrated by means of an electrophoretic variant at the Gpi-B locus. In embryos formed from warmouth x green sunfish hybrid crosses, the paternal GPI-A2 isozymes were first expressed at the same time in both reciprocal hybrids, at 21-25 hr after fertilization. The maternal and paternal GPI-B subunits were synchronously expressed in reciprocal hybrids just for prior to hatching. The parental allelic isozymes at both loci shoed codominant expression in all tissues of the mature F1 hybrids. These results are consistent with the absence of allelic asynchrony and inhibition in interspecific hybrids formed from more evolutionarily related species.
通过淀粉凝胶电泳，在大口黑鲈（Lepomis gulosus）和蓝鳃太阳鱼（L. cyanellus）中分离了位于Gpi - A和 - B位点的磷酸葡萄糖异构酶的等位基因同工酶。这两个物种中GPI - A2、 - AB和 - B2同工酶的特定组织分布和发育表达并无差异。通过Gpi - B位点的电泳变异体，证明了正常种内太阳鱼杂交中等位基因表达的同步性。在大口黑鲈×蓝鳃太阳鱼杂交产生的胚胎中，父本的GPI - A2同工酶在两个正反交杂种中均在受精后21 - 25小时同时首次表达。母本和父本的GPI - B亚基在正反交杂种中刚好在孵化前同步表达。两个位点的亲本等位基因同工酶在成熟F1杂种的所有组织中均表现为共显性表达。这些结果与由亲缘关系更近的物种形成的种间杂种中不存在等位基因异步和抑制现象相一致。
