Pasdar M, Philipp D P, Mohammad W A, Whitt G S
Biochem Genet. 1984 Oct;22(9-10):931-56. doi: 10.1007/BF00499484.
The extent of naturally occurring variations of enzyme locus expression was determined for three tissues (liver, muscle, and eye) in two species of sunfish (Centrarchidae), the green sunfish (Lepomis cyanellus) and the redear sunfish (L. microlophus). The genetic basis for species differences in tissue enzyme specific activities of malate dehydrogenase (EC 1.1.1.37), lactate dehydrogenase (EC 1.1.1.27), phosphoglucomutase (EC 2.7.5.1), and glucosephosphate isomerase (EC 5.3.1.9) was investigated by determining enzyme specific activities in the tissues of the reciprocal F1 hybrids and of their backcross progenies. The specific activities for most enzymes in hybrids were intermediate between those of the parental species. Significant differences in enzyme specific activity were detected among the F1 progeny as well as those of backcrosses. Variations in specific activity levels in one tissue were often independent of variations in specific activities in a different tissue. However, the changes in the specific activities of different enzymes within the same tissue were often positively correlated. The tissue glucosephosphate isomerase activity differences appear not to be due to different functional contributions of the glucosephosphate isomerase allelic isozymes. Cluster analysis of distributions of specific activities revealed no simple Mendelian pattern of inheritance for control of tissue enzyme activity. Our results suggest a polygenic control of tissue enzyme specific activity levels.
测定了两种太阳鱼（鲈形目）——绿太阳鱼（蓝鳃太阳鱼）和红耳太阳鱼组织中三种组织（肝脏、肌肉和眼睛）中酶基因座表达的自然变异程度。通过测定正反交F1杂种及其回交后代组织中的酶比活性，研究了苹果酸脱氢酶（EC 1.1.1.37）、乳酸脱氢酶（EC 1.1.1.27）、磷酸葡萄糖变位酶（EC 2.7.5.1）和葡萄糖磷酸异构酶（EC 5.3.1.9）组织酶比活性物种差异的遗传基础。杂种中大多数酶的比活性介于亲本物种之间。在F1后代以及回交后代中检测到酶比活性的显著差异。一个组织中比活性水平的变化通常与另一个组织中比活性的变化无关。然而，同一组织中不同酶的比活性变化通常呈正相关。组织葡萄糖磷酸异构酶活性差异似乎不是由于葡萄糖磷酸异构酶等位基因同工酶的不同功能贡献。比活性分布的聚类分析显示，组织酶活性的控制不存在简单的孟德尔遗传模式。我们的结果表明组织酶比活性水平受多基因控制。
