Frot-Coutaz J P, de Luca L M
Biochem J. 1976 Dec 1;159(3):799-801. doi: 10.1042/bj1590799.
all-trans-beta-Retinoic acid is phosphorylated to retinoyl phosphate by bis(triethylamine) phosphate with yields of 10-15%. The product is soluble in methanol and is eluted from DEAE-cellulose acetate at a concentration of 0.1M-ammonium acetate in 99% (v/v) methanol. Its phosphate/retinoic acid molar ratio is 1. Retinoyl phosphate has an absorption maximum at 360nm in methanol, whereas retinoic acid has a maximum at 350 nm. The compound is hydrolysed at pH2 and pH13 for 20 min at 37 degrees C, but is relatively stable under the same conditions at pH4, 6, 8 and 10. Retinoyl phosphate (RF 0.1) can be separated from retinyl phosphate (RF 0.2) by chromatography on thin layers of silica gel in chloroform/methanol/water (60:25:4, by vol.).
全反式-β-视黄酸通过磷酸双（三乙胺）磷酸化生成视黄酰磷酸，产率为10 - 15%。产物可溶于甲醇，并在99%（v/v）甲醇中0.1M醋酸铵浓度下从DEAE - 醋酸纤维素上洗脱下来。其磷酸/视黄酸摩尔比为1。视黄酰磷酸在甲醇中的最大吸收波长为360nm，而视黄酸的最大吸收波长为350nm。该化合物在pH2和pH13、37℃下20分钟会水解，但在pH4、6、8和10的相同条件下相对稳定。视黄酰磷酸（比移值0.1）可通过在硅胶薄层上用氯仿/甲醇/水（60:25:4，体积比）进行色谱分离与视黄基磷酸（比移值0.2）分离。
