Barua A B, Olson J A
J Lipid Res. 1985 Oct;26(10):1277-82.
All-trans retinoyl fluoride reacts with the 3,6-lactone of glucuronic acid in slightly alkaline solution to give the lactone of retinoyl beta-glucuronide, along with other retinoyl glucurono-lactones, in about 60% yield. Hydrolysis of the lactone with very dilute alkali gives the free acid, retinoyl beta-glucuronide, in about 80% yield. Pure all-trans retinoyl beta-glucuronide (overall yield: 20-25%) was obtained free from other isomeric and anomeric forms by reverse-phase high pressure liquid chromatography. Retinoyl beta-glucuronide was characterized by UV-visible, infrared, and 1H-NMR spectra, by elementary analysis, by mass spectra, and by its susceptibility to hydrolysis by bacterial beta-glucuronidase.
全反式视黄酰氟在微碱性溶液中与葡萄糖醛酸的3,6-内酯反应，生成视黄酰β-葡萄糖醛酸内酯以及其他视黄酰葡萄糖醛酸内酯，产率约为60%。用极稀的碱水解内酯，得到游离酸视黄酰β-葡萄糖醛酸，产率约为80%。通过反相高压液相色谱法得到了不含其他异构体和异头物形式的纯全反式视黄酰β-葡萄糖醛酸（总产率：20 - 25%）。视黄酰β-葡萄糖醛酸通过紫外可见光谱、红外光谱、1H - NMR光谱、元素分析、质谱以及其对细菌β-葡萄糖醛酸酶水解的敏感性进行表征。
