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仓鼠肝脏微粒体细胞色素P-450酶系统对二甲基亚硝胺去甲基化的磷脂需求

Phospholipid requirement for dimethylnitrosamine demethylation by hamster hepatic microsomal cytochrome P-450 enzyme system.

作者信息

Lotlikar P D, Baldy W J, Nyce J, Dwyer E N

出版信息

Biochem J. 1976 Nov 15;160(2):401-4. doi: 10.1042/bj1600401.

DOI:10.1042/bj1600401
PMID:1008863
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1164247/
Abstract

Extraction with butan-1-ol of freeze-dried microsomal fractions from livers of 3-methyl-cholarthrene-pre-treated hamsters removed about 90% of the total lipid content, but the lipid remaining proved sufficient for the cytochrome P-450 enzyme system to retain about 15-40% of its original catalytic activity for dimethylnitrosamine demethylation. Addition of butan-1-ol-extracted total phospholipid or phosphatidylcholine could not restore any activity, whereas the addition of the synthetic phospholipid dilauroyl phosphatidylcholine was able to restore almost complete activity. Synthetic dipalmitoyl or distearoyl phosphatidylcholine was ineffective in restoring the activity in this reconstituted system.

摘要

用丁醇从经3-甲基胆蒽预处理的仓鼠肝脏冻干微粒体组分中提取，可去除约90%的总脂质含量，但剩余的脂质足以使细胞色素P-450酶系统对二甲基亚硝胺脱甲基反应保留约15 - 40%的原始催化活性。添加丁醇提取的总磷脂或磷脂酰胆碱无法恢复任何活性，而添加合成磷脂二月桂酰磷脂酰胆碱能够恢复几乎完全的活性。合成的二棕榈酰或二硬脂酰磷脂酰胆碱在该重组系统中恢复活性无效。

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Phospholipid requirement for dimethylnitrosamine demethylation by hamster hepatic microsomal cytochrome P-450 enzyme system.仓鼠肝脏微粒体细胞色素P-450酶系统对二甲基亚硝胺去甲基化的磷脂需求
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引用本文的文献

1
Phospholipid requirement for 2-acetamidofluorene N-and ring-hydroxylation by hamster liver microsomal cytochrome P-450 enzyme system.仓鼠肝脏微粒体细胞色素P - 450酶系统对2 - 乙酰氨基芴N - 羟基化和环羟基化的磷脂需求
Biochem J. 1977 Dec 15;168(3):571-4. doi: 10.1042/bj1680571.

本文引用的文献

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Role of phospholipid in electron transfer in a reconstituted liver microsomal enzyme system containing cytochrome P-450.磷脂在含有细胞色素P - 450的重组肝微粒体酶系统电子传递中的作用。
Chem Biol Interact. 1971 Aug;3(4):248-50. doi: 10.1016/0009-2797(71)90046-9.
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Phosphatidylcholine requirement in the enzymatic reduction of hemoprotein P-450 and in fatty acid, hydrocarbon, and drug hydroxylation.
J Biol Chem. 1970 Sep 25;245(18):4851-4.

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