仓鼠肝脏微粒体细胞色素P - 450酶系统对2 - 乙酰氨基芴N - 羟基化和环羟基化的磷脂需求

Phospholipid requirement for 2-acetamidofluorene N-and ring-hydroxylation by hamster liver microsomal cytochrome P-450 enzyme system.

作者信息

Lotlikar P D, Dwyer E N, Baldy W J, Nyce J

出版信息

Biochem J. 1977 Dec 15;168(3):571-4. doi: 10.1042/bj1680571.

DOI:10.1042/bj1680571

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1183807/

Abstract

A phospholipid requirement of 2-acetamidofluorene N- and ring-hydroxylation was investigated with partially delipidated microsomal fraction from livers of 3-methylcholanthrene-pretreated hamsters. Butan-1-ol extraction of microsomal fraction removed 90% of the total lipid content without any appreciable effect on microsomal proteins. Such extracted microsomal fractions had much lower capacity to N- and ring-hydroxylate 2-acetamidofluorene: 25 and 44% of control respectively. Addition of butan-1-ol-extracted total lipid restored both oxidations to some extent, whereas addition of phosphatidylcholine fraction restored both oxidations almost completely. Addition of synthetic phospholipid, dilauroyl phosphatidylcholine, restored both oxidations to a large extent, whereas synthetic dipalmitoyl or distearoyl phosphatidylcholine was ineffective in restoring these oxidations.

摘要

利用来自经3-甲基胆蒽预处理的仓鼠肝脏的部分脱脂微粒体组分，研究了2-乙酰氨基芴N-羟基化和环羟基化对磷脂的需求。用丁醇-1提取微粒体组分可去除90%的总脂质含量，而对微粒体蛋白没有任何明显影响。这种提取的微粒体组分对2-乙酰氨基芴进行N-羟基化和环羟基化的能力要低得多：分别为对照的25%和44%。添加经丁醇-1提取的总脂质可在一定程度上恢复两种氧化反应，而添加磷脂酰胆碱组分几乎可完全恢复两种氧化反应。添加合成磷脂二月桂酰磷脂酰胆碱可在很大程度上恢复两种氧化反应，而合成二棕榈酰或二硬脂酰磷脂酰胆碱在恢复这些氧化反应方面无效。

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本文引用的文献

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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。

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Biochemical formation and pharmacological, toxicological, and pathological properties of hydroxylamines and hydroxamic acids.羟胺和异羟肟酸的生化形成以及药理学、毒理学和病理学特性。

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Inhibitory effect of carbon monoxide on the N- and ring-hydroxylation of 2-acetamidofluorene by hamster hepatic microsomal preparations.一氧化碳对仓鼠肝微粒体制剂中2-乙酰氨基芴的N-羟基化和环羟基化的抑制作用。

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Mol Pharmacol. 1972 Sep;8(5):490-500.

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Role of phospholipid in electron transfer in a reconstituted liver microsomal enzyme system containing cytochrome P-450.磷脂在含有细胞色素P - 450的重组肝微粒体酶系统电子传递中的作用。

Chem Biol Interact. 1971 Aug;3(4):248-50. doi: 10.1016/0009-2797(71)90046-9.

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