Chachra D, Turner C H, Dunipace A J, Grynpas M D
Centre for Biomaterials, University of Toronto, Toronto, Canada.
Calcif Tissue Int. 1999 Apr;64(4):345-51. doi: 10.1007/s002239900630.
Fluoride therapy has been used clinically for many years, but its use remains controversial and many basic questions remain unanswered. Accordingly, this study returns to an animal model to study the effects of high doses of fluoride on bone mineral in rabbits. Twelve rabbits, aged 3(1/2) months at the start of the study, received drinking water fluoridated at 100 ppm while their 12 control counterparts drank distilled water. All rabbits were sacrificed after 6 months. Fluoride was readily incorporated into femoral cortical bone (7473 +/- 966 ppm F versus 1228 +/- 57 ppm in controls; P < 0.00005). Fluoride therapy led to increased mineralization, as measured by density fractionation (P < 0.0005 for the distributions). The bone mineral itself was altered, with a significant increase in the width of crystals (66.2 +/- 2.0 A versus 61.2 +/- 0.9 A; P < 0.01). The microhardness of both cortical and cancellous bone in the femoral head of fluoride-treated rabbits was greater than that in the controls (P < 0.05). The phosphate, calcium, and carbonate contents in the bone was the same in both groups. Finally, fluoride administration did not affect the architecture or connectivity of cancellous bone in the femoral head. Previously published data [1] indicated that the mechanical properties of bone were adversely affected; this suggests that the effect of high doses of fluoride on the strength and stiffness of bone may be mediated by its effect on bone mineral.
氟化物疗法已在临床上应用多年，但其应用仍存在争议，许多基本问题仍未得到解答。因此，本研究回到动物模型，以研究高剂量氟化物对兔骨矿物质的影响。12只兔子在研究开始时年龄为3（1/2）个月，饮用含氟量为100 ppm的氟化水，而它们的12只对照兔子饮用蒸馏水。6个月后所有兔子均被处死。氟化物很容易掺入股骨皮质骨（氟含量为7473±966 ppm，而对照组为1228±57 ppm；P<0.00005）。通过密度分级测量，氟化物疗法导致矿化增加（分布情况P<0.0005）。骨矿物质本身发生了变化，晶体宽度显著增加（66.2±2.0 Å对61.2±0.9 Å；P<0.01）。接受氟化物治疗的兔子股骨头皮质骨和松质骨的显微硬度均大于对照组（P<0.05）。两组骨骼中的磷酸盐、钙和碳酸盐含量相同。最后，给予氟化物并不影响股骨头松质骨的结构或连通性。先前发表的数据[1]表明骨的力学性能受到不利影响；这表明高剂量氟化物对骨强度和刚度的影响可能是由其对骨矿物质的作用介导的。
