Expression, purification, crystallization and preliminary x-ray analysis of the kappa-carrageenase from Pseudoalteromonas carrageenovora.
作者信息
Michel G, Barbeyron T, Flament D, Vernet T, Kloareg B, Dideberg O
机构信息
Laboratoire de Cristallographie Macromoléculaire, Institut de Biologie Structurale Jean-Pierre Ebel, CNRS/CEA, 41 Avenue des Martyrs, 38027 Grenoble CEDEX 1, France.
出版信息
Acta Crystallogr D Biol Crystallogr. 1999 Apr;55(Pt 4):918-20. doi: 10.1107/s0907444998018526.
A recombinant form of His-tagged kappa-carrageenase from Pseudoalteromonas carrageenovora has been expressed, purified and crystallized. Crystals have been obtained by the vapour-diffusion method using polyethylene glycol (Mr = 4000) as a precipitant. These crystals belong to the space group P212121, with unit-cell parameters a = 58.2, b = 62.8, c = 77.9 A, and diffract to 2.2 A resolution on a rotating-anode X-ray source.
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