Sakaue Ryoichi, Nakatsu Toru, Yamaguchi Yoko, Kato Hiroaki, Kajiyama Naoki
Research and Development Division, Kikkoman Corporation, 399 Noda, Noda City, Chiba 278-0037, Japan.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Feb 1;61(Pt 2):196-8. doi: 10.1107/S1744309104034372. Epub 2005 Jan 20.
Bacterial fructosyl amino acid oxidase [fructosyl alpha-L-amino acid:oxygen oxidoreductase (defructosylating); EC 1.5.3] has been crystallized by the hanging-drop vapour-diffusion technique using sodium citrate as the precipitant. Two types of crystals were grown: one type are rhombic prismatic yellow crystals that belong to space group C2 with unit-cell parameters a = 101.08, b = 63.36, c = 83.07 A, beta = 108.80 degrees and diffract to at least 1.8 A resolution, while the second type are rod-like crystals that belong to space group P4(1)22 or P4(3)22 with unit-cell parameters a = b = 119.09, c = 164.66 A and diffract to 2.7 A resolution.
细菌果糖基氨基酸氧化酶[果糖基-α-L-氨基酸：氧氧化还原酶（去果糖基化）；EC 1.5.3]已通过悬滴气相扩散技术，以柠檬酸钠作为沉淀剂进行了结晶。生长出了两种类型的晶体：一种是菱形棱柱形黄色晶体，属于空间群C2，晶胞参数为a = 101.08、b = 63.36、c = 83.07 Å，β = 108.80°，衍射分辨率至少为1.8 Å；而第二种是棒状晶体，属于空间群P4(1)22或P4(3)22，晶胞参数为a = b = 119.09、c = 164.66 Å，衍射分辨率为2.7 Å。
