Sabatino P, Fermani S, Ripamonti A, Cassetta A, Scagliarini S, Trost P
Dipartimento di Chimica 'G. Ciamician', Università di Bologna, via Selmi 2, 40126 Bologna, Italy.
Acta Crystallogr D Biol Crystallogr. 1999 Feb;55(Pt 2):566-7. doi: 10.1107/s090744499801302x.
Glyceraldehyde-3-phosphate dehydrogenase from spinach chloroplasts has been crystallized by vapour diffusion in the pH range 7-8.5 in (NH4)2SO4 and Tris-HCl buffer or potassium phosphate buffer at room temperature. Crystals of the A4 isoform, grown at pH 8.5 in Tris-HCl buffer, diffract to 3.0 A (at 100 K) using synchrotron radiation. The crystals belong to the orthorhombic C222 space group, with unit-cell dimensions a = 145.9, b = 185.9 and c = 106.3 A, and probably contain one tetramer per asymmetric unit. Structure determination by molecular replacement is in progress.
