通过从头计算相位确定的水稻矮缩病毒低分辨率结构。

A low-resolution structure of rice dwarf virus determined by ab initio phasing.

作者信息

Naitow H, Morimoto Y, Mizuno H, Kano H, Omura T, Koizumi M, Tsukihara T

机构信息

The Institute for Protein Research, Osaka University, 3-2 Yamada-oka, Suita 565, Japan.

出版信息

Acta Crystallogr D Biol Crystallogr. 1999 Jan;55(Pt 1):77-84. doi: 10.1107/S0907444998007677. Epub 1999 Jan 1.

DOI:10.1107/S0907444998007677

PMID:10089397

Abstract

Rice dwarf virus crystals belong to space group I222 with cell parameters a = 770 (2), b = 795 (5), c = 814 (5) A and alpha = beta = gamma = 90 degrees. The unit cell of the crystal contains two viruses at the origin and body-centred positions. Using data synthesized from a rice dwarf virus model crystal in the space group I222, the possibility of ab initio phasing was thoroughly examined. The centric nature of the initial phases was unexpectedly broken by extensive iteration of the non-crystallographic symmetry averaging. The structure of rice dwarf virus was then solved with ab initio phasing up to 20 A resolution. The triangulation number determined by the present study is T = 13, which is different from the triangulation number, T = 9, previously determined by electron microscopy [Uyeda & Shikata (1982). Ann. Phytopathol. Soc. Jpn, 48, 295-300].

摘要

水稻矮缩病毒晶体属于空间群I222，晶胞参数为a = 770 (2)，b = 795 (5)，c = 814 (5) Å，α = β = γ = 90°。晶体的晶胞在原点和体心位置包含两个病毒。利用从空间群I222中的水稻矮缩病毒模型晶体合成的数据，对从头相位法的可能性进行了全面研究。通过非晶体学对称平均的广泛迭代，初始相位的中心性质意外地被打破。然后用从头相位法解析了水稻矮缩病毒的结构，分辨率达到20 Å。本研究确定的三角剖分数为T = 13，这与之前通过电子显微镜确定的三角剖分数T = 9不同[Uyeda & Shikata (1982). Ann. Phytopathol. Soc. Jpn, 48, 295 - 300]。