与抗视黄醇结合蛋白（RBP）Fab片段结合的人甲状腺素运载蛋白-视黄醇结合蛋白（RBP）复合物的结晶及初步X射线数据。

Crystallization and preliminary X-ray data for the human transthyretin-retinol-binding protein (RBP) complex bound to an anti-RBP Fab.

作者信息

Malpeli G, Zanotti G, Gliubich F, Rizzotto A, Nishida S K, Folli C, Berni R

机构信息

Institute of Biochemical Sciences, Science Faculty, University of Parma, I-43100 Parma, Italy.

出版信息

Acta Crystallogr D Biol Crystallogr. 1999 Jan;55(Pt 1):276-8. doi: 10.1107/S0907444998007860. Epub 1999 Jan 1.

DOI:10.1107/S0907444998007860

PMID:10089423

Abstract

A macromolecular complex of human transthyretin, human retinol-binding protein and an anti-retinol-binding-protein Fab was crystallized by vapour diffusion in sitting drops. Diffraction from these crystals at cryogenic temperatures was consistent with the space group C222, with cell parameters a = 159.34, b = 222.40 and c = 121.27 A. Crystals diffracted to a resolution limit of 3.36 A using synchrotron radiation. Based on a 2:2:1 stoichiometry for the Fab-retinol-binding-protein-transthyretin complex and the presence of one such complex per asymmetric unit, a reasonable Vm coefficient of 2.74 A3 Da-1 could be estimated.

摘要

人转甲状腺素蛋白、人视黄醇结合蛋白和抗视黄醇结合蛋白Fab的大分子复合物通过坐滴气相扩散法结晶。这些晶体在低温下的衍射与空间群C222一致，晶胞参数a = 159.34、b = 222.40和c = 121.27 Å。使用同步辐射，晶体衍射分辨率极限为3.36 Å。基于Fab-视黄醇结合蛋白-转甲状腺素蛋白复合物的2:2:1化学计量比以及每个不对称单元存在一个这样的复合物，可以估算出合理的Vm系数为2.74 Å3 Da-1。