一种单克隆抗体Fab片段，在有和没有来自HIV的肽表位的情况下均结晶。

A monoclonal antibody Fab fragment crystallized with and without a peptide epitope from HIV.

作者信息

Pfeffer-Hennig S, Wessner H, Hennig M, Dauter Z, Hausdorf G, Höhne W E

机构信息

Institute of Biochemistry, Humboldt University, Berlin, Germany.

出版信息

Proteins. 1993 Aug;16(4):437-9. doi: 10.1002/prot.340160411.

DOI:10.1002/prot.340160411

PMID:7689218

Abstract

The Fab fragment of CB 4-1, a monoclonal murine antibody against HIV protein p24, has been produced. It forms a complex with a synthetic antigen, an epitope of p24 made up of 11 amino acids, with the binding constant Kd = 3.6 x 10(-9) M. Crystals of hexagonal and orthorhombic space group has been obtained by cocrystallization of the Fab with the epitope and crystallization without the epitope, respectively. In either case, the crystals are suitable for X-ray structural analysis. Crystals of the Fab fragment cocrystallized with the peptide have the space group P 6(3)22 with cell dimensions of a = b = 105 A, c = 297 A. Fab crystals without the epitope are in space group C 222 with cell dimensions a = 110.1 A, b = 110.2 A, c = 150.1 A.

摘要

已制备出抗HIV蛋白p24的单克隆鼠抗体CB 4-1的Fab片段。它与一种合成抗原（由11个氨基酸组成的p24表位）形成复合物，结合常数Kd = 3.6×10⁻⁹ M。通过分别将Fab与表位共结晶以及在无表位情况下结晶，获得了六方和正交空间群的晶体。在这两种情况下，晶体都适合进行X射线结构分析。与肽共结晶的Fab片段晶体的空间群为P 6₃22，晶胞参数a = b = 105 Å，c = 297 Å。无表位的Fab晶体属于空间群C 222，晶胞参数a = 110.1 Å，b = 110.2 Å，c = 150.1 Å。