Zhou M, Pugmire M J, Vuong B Q, Ealick S E
Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, NY 14853, USA.
Acta Crystallogr D Biol Crystallogr. 1999 Jan;55(Pt 1):287-90. doi: 10.1107/S090744499800852X. Epub 1999 Jan 1.
Pyrimidine nucleoside phosphorylase (PYNP) from B. stearothermophilus has been cloned and purified for crystallization. Crystals of a potential protein-inhibitor complex have been prepared by co-crystallization techniques using the substrate analog pseudouridine. These crystals provide good-quality diffraction images to 2.7 A and belong to space group P21. The asymmetric unit contains the dimer structure of PYNP with unit-cell parameters a = 53.9, b = 71.9, c = 123.3 A and beta = 96.9 degrees.
嗜热栖热菌的嘧啶核苷磷酸化酶（PYNP）已被克隆并纯化用于结晶。通过使用底物类似物假尿苷的共结晶技术制备了潜在的蛋白质-抑制剂复合物晶体。这些晶体提供了分辨率达2.7埃的高质量衍射图像，属于空间群P21。不对称单元包含PYNP的二聚体结构，晶胞参数为a = 53.9、b = 71.9、c = 123.3埃，β = 96.9°。
