Conkie J A, Davidson J F
Thromb Haemost. 1978 Jun 30;39(3):564-73.
Antiserum to the carboxymethylated Aalpha chain of human fibrinogen has been produced in rabbits and its reactions investigated in a double diffusion system. The antiserum reacts with the carboxymethylated Aalpha chains, but not with carboxymethylated gamma chains, nor with carboxymethylated Bbeta chains, provided that the antiserum had been absorbed with carboxymethylated Bbeta chains. The antiserum also reacts with Aalpha chains in the whole fibrinogen molecule, either in plasma or in the purified form, and spur formation was observed when intact fibrinogen was compared with high solubility fibrinogen, which contained little or no intact Aalpha chain.
已在兔体内制备了针对人纤维蛋白原羧甲基化Aα链的抗血清，并在双向扩散系统中研究了其反应。该抗血清能与羧甲基化Aα链发生反应，但不与羧甲基化γ链反应，也不与羧甲基化Bβ链反应，前提是该抗血清已用羧甲基化Bβ链进行了吸收处理。该抗血清还能与血浆或纯化形式的完整纤维蛋白原分子中的Aα链发生反应，当将完整纤维蛋白原与几乎不含或不含完整Aα链的高溶解性纤维蛋白原进行比较时，观察到了刺状物的形成。
