Kataoka M, Yamamoto K, Shimizu S, Ohta M, Kita A, Higuchi Y, Miki K
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan.
Acta Crystallogr D Biol Crystallogr. 1998 Nov 1;54(Pt 6 Pt 2):1432-4. doi: 10.1107/s0907444998006131.
The lactonohydrolase from the fungus Fusarium oxysporum AKU 3702 was crystallized by the vapour-diffusion procedure in the presence of polyethylene glycol 4000 as a precipitant. The crystals belong to the monoclinic space group P21 with unit-cell parameters a = 156, b = 100, c = 94.1 A, beta = 91.7 degrees. Assuming that there are two lactonohydrolase-dimer molecules in the asymmetric unit, the crystal volume per unit molecular mass, Vm, is calculated to be 2.94 A3 Da-1.
来自尖孢镰刀菌AKU 3702的内酯水解酶通过气相扩散法在聚乙二醇4000作为沉淀剂的情况下进行结晶。晶体属于单斜空间群P21，晶胞参数为a = 156、b = 100、c = 94.1 Å、β = 91.7°。假设不对称单元中有两个内酯水解酶二聚体分子，每单位分子量的晶体体积Vm经计算为2.94 ų Da⁻¹。
