荧光假单胞菌羧酸酯酶的结晶及初步X射线晶体学分析

Crystallization and preliminary X-ray crystallographic analysis of carboxylesterase from pseudomonas fluorescens.

作者信息

Kim K K, Hwang K Y, Jang W H, Lee H, Yoo O J, Choi M U, Suh S W

机构信息

Department of Chemistry, College of Natural Sciences, Seoul National University, Korea.

出版信息

Arch Biochem Biophys. 1993 May;302(2):417-9. doi: 10.1006/abbi.1993.1233.

DOI:10.1006/abbi.1993.1233

PMID:8489246

Abstract

Large crystals of carboxylesterase from Pseudomonas fluorescens have been grown in the presence of dioxane using ammonium sulfate and lithium sulfate as precipitant. They belong to the tetragonal space group P4(1)2(1)2 (or P4(3)2(1)2), with unit cell dimensions of a = 82.01 (+/-0.06) A and c = 145.44 (+/- 0.08) A. The presence of one carboxylesterase dimer in the asymmetric unit gives the crystal volume per protein mass (VM) of 2.56 A3/Da and solvent fraction of 52.0% by volume. The crystals diffract to at least 2.3 A Bragg spacing when exposed to CuK alpha X rays from a rotating anode generator. X-ray data (nearly complete to 2.6 A Bragg spacing) have been collected from a native crystal.

摘要

荧光假单胞菌羧酸酯酶的大晶体已在二氧六环存在的情况下，使用硫酸铵和硫酸锂作为沉淀剂生长出来。它们属于四方晶系空间群P4(1)2(1)2（或P4(3)2(1)2），晶胞参数为a = 82.01（±0.06）Å，c = 145.44（±0.08）Å。不对称单元中存在一个羧酸酯酶二聚体，使得每蛋白质质量的晶体体积（VM）为2.56 Å3/Da，溶剂体积分数为52.0%。当暴露于旋转阳极发生器产生的CuKα X射线时，这些晶体的衍射至少可达2.3 Å的布拉格间距。已从一个天然晶体收集了X射线数据（几乎完整到2.6 Å的布拉格间距）。